Name: U-Blot® LEF1 Rabbit mAb
SKU: WR4213
Price: 268 USD
Availability: InStock

Product details

Background:This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009],

Specifications

Size	50μL/100μL
Target	LEF-1
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, IP, ELISA
MW(Calculated)	44kD
MW(Observed)	45-60kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:2000-1:5000; WB 1:1000-1:5000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0601R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	LEF1
Protein Name	Lymphoid enhancer-binding factor 1
Other Name	LEF1; / Lymphoid enhancer-binding factor 1; / LEF-1; / T cell-specific transcription factor 1-alpha; / TCF1-alpha
Species	Human
Gene ID	5604
Uniprot	Q9UJU2,
Species.1	Mouse
Gene ID.1	16842
Uniprot.1	P27782,
Species.2	Rat
Gene ID.2	170851
Uniprot.2	Q01986
Cellular Localization	Nucleus
Tissue Expression	Widely expressed, with extremely low levels in brain.
Signaling_pathway	Cellular Processes >> Transport and catabolism >> Autophagy - animal;Cellular Processes >> Cell growth and death >> Apoptosis;Cellular Processes >> Cell growth and death >> Cellular senescence;Cellular Processes >> Cellular community - eukaryotes >> Focal adhesion;Cellular Processes >> Cellular community - eukaryotes >> Gap junction;Cellular Processes >> Cellular community - eukaryotes >> Signaling pathways regulating pluripotency of stem cells;Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Organismal Systems >> Immune system >> Neutrophil extracellular trap formation;Organismal Systems >> Immune system >> Toll-like receptor signaling pathway;Organismal Systems >> Immune system >> Natural killer cell mediated cytotoxicity;Organismal Systems >> Immune system >> T cell receptor signaling pathway;Organismal Systems >> Immune system >> B cell receptor signaling pathway;Organismal Systems >> Immune system >> Fc epsilon RI signaling pathway;Organismal Systems >> Immune system >> Fc gamma R-mediated phagocytosis;Organismal Systems >> Immune system >> Chemokine signaling pathway;Organismal Systems >> Endocrine system >> Insulin signaling pathway;Organismal Systems >> Endocrine system >> GnRH signaling pathway;Organismal Systems >> Endocrine system >> Estrogen signaling pathway;Organismal Systems >> Endocrine system >> Prolactin signaling pathway;Organismal Systems >> Endocrine system >> Oxytocin signaling pathway;Organismal Systems >> Endocrine system >> Relaxin signaling pathway;Organismal Systems >> Endocrine system >> Growth hormone synthesis, secretion and action;Organismal Systems >> Endocrine system >> Thyroid hormone signaling pathway;Organismal Systems >> Circulatory system >> Vascular smooth muscle contraction;Organismal Systems >> Nervous system >> Cholinergic synapse;Organismal Systems >> Nervous system >> Neurotrophin signaling pathway;Organismal Systems >> Development and regeneration >> Osteoclast differentiation;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: overview >> MicroRNAs in cancer;Human Diseases >> Cancer: overview >> Central carbon metabolism in cancer;Human Diseases >> Cancer: overview >> PD-L1 expression and PD-1 checkpoint pathway in cancer;Human Diseases >> Cancer: specific types >> Colorectal cancer;Human Diseases >> Cancer: specific types >> Pancreatic cancer;Human Diseases >> Cancer: specific types >> Hepatocellular carcinoma;Human Diseases >> Cancer: specific types >> Gastric cancer;Human Diseases >> Cancer: specific types >> Glioma;Human Diseases >> Cancer: specific types >> Thyroid cancer;Human Diseases >> Cancer: specific types >> Acute myeloid leukemia;Human Diseases >> Cancer: specific types >> Chronic myeloid leukemia;Human Diseases >> Cancer: specific types >> Melanoma;Human Diseases >> Cancer: specific types >> Renal cell carcinoma;Human Diseases >> Cancer: specific types >> Bladder cancer;Human Diseases >> Cancer: specific types >> Prostate cancer;Human Diseases >> Cancer: specific types >> Endometrial cancer;Human Diseases >> Cancer: specific types >> Breast cancer;Human Diseases >> Cancer: specific types >> Non-small cell lung cancer;Human Diseases >> Neurodegenerative disease >> Alzheimer disease;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> ErbB signaling pathway;Environmental Information Processing >> Signal transduction >> Ras signaling pathway;Environmental Information Processing >> Signal transduction >> Rap1 signaling pathway;Environmental Information Processing >> Signal transduction >> VEGF signaling pathway;Environmental Information Processing >> Signal transduction >> Apelin signaling pathway;Environmental Information Processing >> Signal transduction >> TNF signaling pathway;Environmental Information Processing >> Signal transduction >> HIF-1 signaling pathway;Environmental Information Processing >> Signal transduction >> FoxO signaling pathway;Environmental Information Processing >> Signal transduction >> Phospholipase D signaling pathway;Environmental Information Processing >> Signal transduction >> Sphingolipid signaling pathway;Environmental Information Processing >> Signal transduction >> cAMP signaling pathway;Environmental Information Processing >> Signal transduction >> cGMP-PKG signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway;Environmental Information Processing >> Signal transduction >> mTOR signaling pathway
Research Areas	>>EGFR tyrosine kinase inhibitor resistance; / >>Endocrine resistance; / >>MAPK signaling pathway; / >>ErbB signaling pathway; / >>Ras signaling pathway; / >>Rap1 signaling pathway; / >>cGMP-PKG signaling pathway; / >>cAMP signaling pathway; / >>Chemokine signaling pathway; / >>HIF-1 signaling pathway; / >>FoxO signaling pathway; / >>Sphingolipid signaling pathway; / >>Phospholipase D signaling pathway; / >>Oocyte meiosis; / >>Autophagy - animal; / >>mTOR signaling pathway; / >>PI3K-Akt signaling pathway; / >>Apoptosis; / >>Cellular senescence; / >>Vascular smooth muscle contraction; / >>VEGF signaling pathway; / >>Apelin signaling pathway; / >>Osteoclast differentiation; / >>Focal adhesion; / >>Gap junction; / >>Signaling pathways regulating pluripotency of stem cells; / >>Neutrophil extracellular trap formation; / >>Toll-like receptor signaling pathway; / >>Natural killer cell mediated cytotoxicity; / >>T cell receptor signaling pathway; / >>B cell receptor signaling pathway; / >>Fc epsilon RI signaling pathway; / >>Fc gamma R-mediated phagocytosis; / >>TNF signaling pathway; / >>Long-term potentiation; / >>Neurotrophin signaling pathway; / >>Cholinergic synapse; / >>Serotonergic synapse; / >>Long-term depression; / >>Regulation of actin cytoskeleton; / >>Insulin signaling pathway; / >>GnRH signaling pathway; / >>Progesterone-mediated oocyte maturation; / >>Estrogen signaling pathway; / >>Melanogenesis; / >>Prolactin signaling pathway; / >>Thyroid hormone signaling pathway; / >>Oxytocin signaling pathway; / >>Relaxin signaling pathway; / >>Parathyroid hormone synthesis, secretion and action; / >>GnRH secretion; / >>Cushing syndrome; / >>Growth hormone synthesis, secretion and action; / >>Alzheimer disease; / >>Pathways of neurodegeneration - multiple diseases; / >>Alcoholism; / >>Salmonella infection; / >>Yersinia infection; / >>Hepatitis C; / >>Hepatitis B; / >>Human cytomegalovirus infection; / >>Influenza A; / >>Human papillomavirus infection; / >>Human T-cell leukemia virus 1 infection; / >>Kaposi sarcoma-associated herpesvirus infection; / >>Human immunodeficiency virus 1 infection; / >>Pathways in cancer; / >>Proteoglycans in cancer; / >>MicroRNAs in cancer; / >>Chemical carcinogenesis - receptor activation; / >>Chemical carcinogenesis - reactive oxygen species; / >>Colorectal cancer; / >>Renal cell carcinoma; / >>Pancreatic cancer; / >>Endometrial cancer; / >>Glioma; / >>Prostate cancer; / >>Thyroid cancer; / >>Melanoma; / >>Bladder cancer; / >>Chronic myeloid leukemia; / >>Acute myeloid leukemia; / >>Non-small cell lung cancer; / >>Breast cancer; / >>Hepatocellular carcinoma; / >>Gastric cancer; / >>Central carbon metabolism in cancer; / >>Choline metabolism in cancer; / >>PD-L1 expression and PD-1 checkpoint pathway in cancer
Function	Alternative products:Additional isoforms seem to exist,Domain:Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors.,Function:Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling.,similarity:Belongs to the TCF/LEF family.,similarity:Contains 1 HMG box DNA-binding domain.,subcellular location:Found in nuclear bodies upon PIASG binding.,subunit:Binds the armadillo repeat of CTNNB1 and forms a stable complex. Interacts with EP300, TLE1 and PIASG (By similarity). Binds THOC4, MDFI and MDFIC.,tissue specificity:Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines.,