Price range: $268.00 through $328.00
Background:
This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009],
| Size | 50μL/100μL |
| Target | LSD1 |
| Reactivity | Human, Mouse, Bovine, Dog, Pig |
| Applications | WB, IHC, IF |
| MW(Calculated) | 93kD |
| MW(Observed) | |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:1000-1:2000; IHC 1:500-1:1000; IF 1:100-1:500; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | Affinity purification |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 11D12 |
| Immunogen | Purified recombinant human LSD1 (N-terminus) protein fragments expressed in E.coli. |
| Specificity | LSD1 Monoclonal Antibody detects endogenous levels of LSD1 protein. |
| Gene Name | KDM1A AOF2 KDM1 KIAA0601 LSD1 |
| Protein Name | Lysine-specific histone demethylase 1A |
| Other Name | KDM1A; / AOF2; / KDM1; / KIAA0601; / LSD1; / Lysine-specific histone demethylase 1A; / BRAF35-HDAC complex protein BHC110; / Flavin-containing amine oxidase domain-containing protein 2 |
| Species | Human |
| Gene ID | 23028 |
| Uniprot | O60341, |
| Species.1 | Mouse |
| Gene ID.1 | 99982 |
| Uniprot.1 | Q6ZQ88 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus . |
| Tissue Expression | Ubiquitously expressed. |
| Signaling_pathway | |
| Research Areas | >>Thermogenesis |
| Function | cofactor:FAD.,Domain:The SWIRN domain may act as an anchor site for a histone tail.,Function:Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. May also demethylate 'Lys-9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis.,similarity:Belongs to the flavin monoamine oxidase family.,similarity:Contains 1 SWIRM domain.,subunit:Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B/BRAF35, KDM1, RCOR1/CoREST and PHF21A/BHC80. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. In the complex, RCOR1/CoREST strongly enhances the demethylase activity and protects it from the proteasome while PHF21A/BHC80 inhibits the demethylase activity. Interacts with the androgen receptor (AR).,tissue specificity:Ubiquitously expressed., |
