Price range: $268.00 through $328.00
Background:
The protein encoded by this gene is a member of serine/threonine protein kinase family. This kinase contains a dual leucine-zipper motif, and has been shown to form dimers/oligomers through its leucine-zipper motif. This kinase can phosphorylate and activate MAPK8/JNK, MAP2K7/MKK7, which suggests a role in the JNK signaling pathway. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | LZK |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 108kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human M3K13. AA range:151-200 |
| Specificity | LZK Polyclonal Antibody detects endogenous levels of LZK protein. |
| Gene Name | MAP3K13 |
| Protein Name | Mitogen-activated protein kinase kinase kinase 13 |
| Other Name | MAP3K13; / LZK; / Mitogen-activated protein kinase kinase kinase 13; / Leucine zipper-bearing kinase; / Mixed lineage kinase; / MLK |
| Species | Human |
| Gene ID | 9175 |
| Uniprot | O43283, |
| Species.1 | Mouse |
| Gene ID.1 | 71751 |
| Uniprot.1 | Q1HKZ5 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm . Membrane; Peripheral membrane protein . |
| Tissue Expression | Expressed in the adult brain, liver, placenta and pancreas, with expression strongest in the pancreas. |
| Signaling_pathway | Environmental Information Processing >> Signal transduction >> MAPK signaling pathway |
| Research Areas | >>MAPK signaling pathway |
| Function | Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Activated by autophosphorylation and homodimerization.,Function:Activates the JUN N-terminal pathway through activation of the MAP kinase kinase MAP2K7. Acts synergistically with PRDX3 to regulate the activation of NF-kappa-B in the cytosol. This activation is kinase-dependent and involves activating the IKK complex, the IKBKB-containing complex that phosphorylates inhibitors of NF-kappa-B.,PTM:Autophosphorylated on serine and threonine residues.,sequence Caution:Translated as Tyr.,sequence Caution:Wrong choice of CDS.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Homodimer; forms dimers through the leucine-zipper motif. Interacts with the C-terminus of MAPK8IP1 through the kinase catalytic domain. Binds PRDX3. Associates with the IKK complex through the kinase domain.,tissue specificity:Expressed in the adult brain, liver, placenta and pancreas, with expression strongest in the pancreas., |
