MLC-2 Polyclonal Antibody
Ucallm_WP13184-50
Price range: $268.00 through $328.00
Product details
Background:
This gene encodes a nonsarcomeric myosin regulatory light chain. This protein is activated by phosphorylation and regulates smooth muscle and non-muscle cell contraction. This protein may also be involved in DNA damage repair by sequestering the transcriptional regulator apoptosis-antagonizing transcription factor (AATF)/Che-1 which functions as a repressor of p53-driven apoptosis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 8.[provided by RefSeq, Dec 2014],
Specifications
| Size | 50μL/100μL |
| Target | MLC-2 |
| Reactivity | Human, Mouse, Rat |
| Applications | IHC, IF, ELISA |
| MW(Calculated) | 20kD |
| MW(Observed) | |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:5000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MLC-2. AA range:5-54 |
| Specificity | MLC-2 Polyclonal Antibody detects endogenous levels of MLC-2 protein. |
| Gene Name | MYL12A |
| Protein Name | Myosin regulatory light chain 12A |
| Other Name | MYL12A; / MLCB; / MRLC3; / RLC; / Myosin regulatory light chain 12A; / MLC-2B; / Myosin RLC; / Myosin regulatory light chain 2; / nonsarcomeric; / Myosin regulatory light chain MRLC3; / MYL12B; / MRLC2; / MYLC2B; / Myosin regulatory light chain 12B; / MLC-2A; / MLC-2; / M |
| Species | Human |
| Gene ID | 10398;10627 |
| Uniprot | P19105;O14950;P24844, |
| Species.1 | Mouse |
| Gene ID.1 | 67938;98932, |
| Uniprot.1 | |
| Species.2 | Rat |
| Gene ID.2 | 50685;296313 |
| Uniprot.2 | P18666;Q64122 |
| Cellular Localization | stress fiber,cytosol,brush border,myosin complex,myosin II complex,Z disc,apical part of cell,extracellular exosome, |
| Tissue Expression | Coronary artery,Epithelium,Placenta,Skeletal muscle,Skin,Testis, |
| Signaling_pathway | Cellular Processes >> Cellular community - eukaryotes >> Focal adhesion;Cellular Processes >> Cellular community - eukaryotes >> Adherens junction;Cellular Processes >> Cellular community - eukaryotes >> Tight junction;Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Organismal Systems >> Immune system >> Platelet activation;Organismal Systems >> Immune system >> Leukocyte transendothelial migration;Organismal Systems >> Endocrine system >> Oxytocin signaling pathway;Organismal Systems >> Circulatory system >> Vascular smooth muscle contraction;Environmental Information Processing >> Signal transduction >> cAMP signaling pathway;Environmental Information Processing >> Signal transduction >> cGMP-PKG signaling pathway |
| Research Areas | >>Axon guidance; / >>Focal adhesion; / >>Tight junction; / >>Platelet activation; / >>Leukocyte transendothelial migration; / >>Regulation of actin cytoskeleton; / >>Shigellosis; / >>Salmonella infection |
| Function | Function:Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.,miscellaneous:This chain binds calcium.,PTM:Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.,similarity:Contains 3 EF-hand domains.,subunit:Myosin is an hexamer of 2 heavy chains and 4 light chains., |
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