Price range: $268.00 through $328.00
Background:
This gene encodes a homeobox-containing transcription factor. This transcription factor functions as a negative regulator of epithelial cell growth in prostate tissue. Aberrant expression of this gene is associated with prostate tumor progression. Alternate splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jan 2012],
| Size | 50μL/100μL |
| Target | NKX3.1 |
| Reactivity | Human |
| Applications | WB, IHC, IF, FC, ELISA |
| MW(Calculated) | 26kD |
| MW(Observed) | |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:200-1:1000; Flow Cyt 1:200-1:400; ELISA 1:10000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | Affinity purification |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 13E5 |
| Immunogen | Purified recombinant fragment of human Nkx-3.1 expressed in E. Coli. |
| Specificity | Nkx-3.1 Monoclonal Antibody detects endogenous levels of Nkx-3.1 protein. |
| Gene Name | NKX3-1 NKX3.1 NKX3A |
| Protein Name | Homeobox protein Nkx-3.1 |
| Other Name | NKX3-1; / NKX3.1; / NKX3A; / Homeobox protein Nkx-3.1; / Homeobox protein NK-3 homolog A |
| Species | Human |
| Gene ID | 4824 |
| Uniprot | Q99801, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | P97436 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus . |
| Tissue Expression | Highly expressed in the prostate and, at a lower level, in the testis. |
| Signaling_pathway | Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: specific types >> Prostate cancer |
| Research Areas | >>Pathways in cancer; / >>Prostate cancer |
| Function | Alternative products:Additional isoforms seem to exist,Disease:NKX3-1 has been thought to be one of the target gene of the 8p21 loss of heterozygosity, common in prostate cancer, but neither disruption of the coding region of the gene, nor mutations have been found in prostate cancer.,Function:Transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. Could play an important role in regulating proliferation of glandular epithelium and in the formation of ducts in prostate.,induction:By androgens and, in the LNCAP cell line, by estrogens. Androgenic control may be lost in prostate cancer cells during tumor progression from an androgen-dependent to an androgen-independent phase.,similarity:Belongs to the NK-3 homeobox family.,similarity:Contains 1 homeobox DNA-binding domain.,subunit:Interacts with serum response factor (SRF) (By similarity). Interacts with SPDEF. Interacts with WDR77.,tissue specificity:Highly expressed in the prostate and, at a lower level, in the testis., |
