U-Blot® PDK1 Rabbit mAb
Ucallm_WR5034-50
Price range: $268.00 through $328.00
Product details
Background:Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2013],
Specifications
| Size | 50μL/100μL |
| Target | PDK1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IF, IP, ELISA |
| MW(Calculated) | 49kD |
| MW(Observed) | 49kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1266R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | PDK1 |
| Protein Name | [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1 mitochondrial |
| Other Name | PDK1; / PDHK1; / [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1; / mitochondrial; / Pyruvate dehydrogenase kinase isoform 1 |
| Species | Human |
| Gene ID | 5163 |
| Uniprot | Q15118, |
| Species.1 | Mouse |
| Gene ID.1 | 228026 |
| Uniprot.1 | Q8BFP9, |
| Species.2 | Rat |
| Gene ID.2 | |
| Uniprot.2 | Q63065 |
| Cellular Localization | Mitochondrion matrix . |
| Tissue Expression | Expressed predominantly in the heart. Detected at lower levels in liver, skeletal muscle and pancreas. |
| Signaling_pathway | Human Diseases >> Cancer: overview >> Central carbon metabolism in cancer;Environmental Information Processing >> Signal transduction >> HIF-1 signaling pathway |
| Research Areas | >>HIF-1 signaling pathway; / >>Central carbon metabolism in cancer |
| Function | Catalytic activity:ATP + [pyruvate dehydrogenase (acetyl-transferring)] = ADP + [pyruvate dehydrogenase (acetyl-transferring)] phosphate.,Function:Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.,similarity:Belongs to the PDK/BCKDK protein kinase family.,similarity:Contains 1 histidine kinase domain.,tissue specificity:Expressed predominantly in the heart., |
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