Name: PKC [PTR1190] mouse mAb
SKU: WM1250
Price: 268 USD
Availability: InStock

Product details

Background:
protein kinase C alpha(PRKCA) Homo sapiens Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq, Jul 2

Specifications

Size	50μL/100μL
Target	PRKCA
Reactivity	Human, Mouse, Rat, Bovine
Applications	WB, IF, ELISA
MW(Calculated)
MW(Observed)	80kD
Host Species	Mouse
Isotype	IgG
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:500-2000; IF 1:100-500; ELISA 1:1000-5000
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein G affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Monoclonal
Clone Number	RMMR1190
Immunogen	Synthesized peptide derived from human PKC AA range: 450-550
Specificity	This antibody detects endogenous levels of PKC protein.
Gene Name	PRKCA PKCA PRKACA
Protein Name	Protein kinase C alpha type (PKC-A) (PKC-alpha) (EC 2.7.11.13)
Other Name
Species	Human
Gene ID	5578
Uniprot	P17252,
Species.1	Mouse
Gene ID.1	18750
Uniprot.1	P20444
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Cytoplasmic
Tissue Expression
Signaling_pathway	Cellular Processes >> Cellular community - eukaryotes >> Focal adhesion;Cellular Processes >> Cellular community - eukaryotes >> Gap junction;Organismal Systems >> Immune system >> Neutrophil extracellular trap formation;Organismal Systems >> Immune system >> Natural killer cell mediated cytotoxicity;Organismal Systems >> Immune system >> Fc epsilon RI signaling pathway;Organismal Systems >> Immune system >> Fc gamma R-mediated phagocytosis;Organismal Systems >> Immune system >> Leukocyte transendothelial migration;Organismal Systems >> Endocrine system >> Insulin secretion;Organismal Systems >> Endocrine system >> GnRH signaling pathway;Organismal Systems >> Endocrine system >> Oxytocin signaling pathway;Organismal Systems >> Endocrine system >> Relaxin signaling pathway;Organismal Systems >> Endocrine system >> Growth hormone synthesis, secretion and action;Organismal Systems >> Endocrine system >> Thyroid hormone signaling pathway;Organismal Systems >> Endocrine system >> Aldosterone synthesis and secretion;Organismal Systems >> Circulatory system >> Adrenergic signaling in cardiomyocytes;Organismal Systems >> Circulatory system >> Vascular smooth muscle contraction;Organismal Systems >> Nervous system >> Glutamatergic synapse;Organismal Systems >> Nervous system >> Cholinergic synapse;Organismal Systems >> Nervous system >> Dopaminergic synapse;Organismal Systems >> Sensory system >> Inflammatory mediator regulation of TRP channels;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: overview >> MicroRNAs in cancer;Human Diseases >> Cancer: specific types >> Hepatocellular carcinoma;Human Diseases >> Cancer: specific types >> Glioma;Human Diseases >> Cancer: specific types >> Non-small cell lung cancer;Human Diseases >> Neurodegenerative disease >> Spinocerebellar ataxia;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> ErbB signaling pathway;Environmental Information Processing >> Signal transduction >> Ras signaling pathway;Environmental Information Processing >> Signal transduction >> Rap1 signaling pathway;Environmental Information Processing >> Signal transduction >> Wnt signaling pathway;Environmental Information Processing >> Signal transduction >> VEGF signaling pathway;Environmental Information Processing >> Signal transduction >> HIF-1 signaling pathway;Environmental Information Processing >> Signal transduction >> Calcium signaling pathway;Environmental Information Processing >> Signal transduction >> Phospholipase D signaling pathway;Environmental Information Processing >> Signal transduction >> Sphingolipid signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway;Environmental Information Processing >> Signal transduction >> mTOR signaling pathway
Research Areas
Function	Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in positive and negative regulation of cell proliferation, apoptosis, differentiation, migration and adhesion, tumorigenesis, cardiac hypertrophy, angiogenesis, platelet function and inflammation, by directly phosphorylating targets such as RAF1, BCL2, CSPG4, TNNT2/CTNT, or activating signaling cascade involving MAPK1/3 (ERK1/2) and RAP1GAP. Involved in cell proliferation and cell growth arrest by positive and negative regulation of the cell cycle. Can promote cell growth by phosphorylating and activating RAF1, which mediates the activation of the MAPK/ERK signaling cascade, and/or by up-regulating CDKN1A, which facilitates active cyclin-dependent kinase (CDK) complex formation in glioma cells. In intestinal cells stimulated by the phorbol ester PMA, can trigger a cell cycle arrest program which is associated with the accumulation of the hyper-phosphorylated growth-suppressive form of RB1 and induction of the CDK inhibitors CDKN1A and CDKN1B. Exhibits anti-apoptotic function in glioma cells and protects them from apoptosis by suppressing the p53/TP53-mediated activation of IGFBP3, and in leukemia cells mediates anti-apoptotic action by phosphorylating BCL2. During macrophage differentiation induced by macrophage colony-stimulating factor (CSF1), is translocated to the nucleus and is associated with macrophage development. After wounding, translocates from focal contacts to lamellipodia and participates in the modulation of desmosomal adhesion. Plays a role in cell motility by phosphorylating CSPG4, which induces association of CSPG4 with extensive lamellipodia at the cell periphery and polarization of the cell accompanied by increases in cell motility. During chemokine-induced CD4(+) T cell migration, phosphorylates CDC42-guanine exchange factor DOCK8 resulting in its dissociation from LRCH1 and the activation of GTPase CDC42 . Is highly expressed in a number of cancer cells where it can act as a tumor promoter and is implicated in malignant phenotypes of several tumors such as gliomas and breast cancers. Negatively regulates myocardial contractility and positively regulates angiogenesis, platelet aggregation and thrombus formation in arteries. Mediates hypertrophic growth of neonatal cardiomyocytes, in part through a MAPK1/3 (ERK1/2)-dependent signaling pathway, and upon PMA treatment, is required to induce cardiomyocyte hypertrophy up to heart failure and death, by increasing protein synthesis, protein-DNA ratio and cell surface area. Regulates cardiomyocyte function by phosphorylating cardiac troponin T (TNNT2/CTNT), which induces significant reduction in actomyosin ATPase activity, myofilament calcium sensitivity and myocardial contractility. In angiogenesis, is required for full endothelial cell migration, adhesion to vitronectin (VTN), and vascular endothelial growth factor A (VEGFA)-dependent regulation of kinase activation and vascular tube formation. Involved in the stabilization of VEGFA mRNA at post-transcriptional level and mediates VEGFA-induced cell proliferation. In the regulation of calcium-induced platelet aggregation, mediates signals from the CD36/GP4 receptor for granule release, and activates the integrin heterodimer ITGA2B-ITGB3 through the RAP1GAP pathway for adhesion. During response to lipopolysaccharides (LPS), may regulate selective LPS-induced macrophage functions involved in host defense and inflammation. But in some inflammatory responses, may negatively regulate NF-kappa-B-induced genes, through IL1A-dependent induction of NF-kappa-B inhibitor alpha (NFKBIA/IKBA). Upon stimulation with 12-O-tetradecanoylphorbol-13-acetate (TPA), phosphorylates EIF4G1, which modulates EIF4G1 binding to MKNK1 and may be involved in the regulation of EIF4E phosphorylation. Phosphorylates KIT, leading to inhibition of KIT activity. Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription. Phosphorylates SOCS2 at 'Ser-52' facilitating its ubiquitination and proteosomal degradation (By similarity).

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PKC [PTR1190] mouse mAb

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PKC [PTR1190] mouse mAb

Product details

Specifications

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