SKU: WP15515 Categories: ,

POLR3G Polyclonal Antibody

Price range: $268.00 through $328.00

SKU: WP15515-100
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Product details

Background:

function:DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the RPC3/POLR3C-RPC6/POLR3F-RPC7/POLR3G subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation., similarity:Belongs to the eukaryotic RPC7 RNA polymerase subunit family., subunit:Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits (By similarity). RPC3/POLR3C, RPC6/POLR3F and RPC7/POLR3G form a Pol III subcomplex.,

Specifications

TargetPOLR3G
ReactivityHuman, Mouse
ApplicationsWB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)26kD
Host SpeciesRabbit
IsotypeIgG
Conjugate
ModificationUnmodified
Recommended Dilution RatioWB 1:500-1:2000;IHC: 1:100-1:300;ELISA 1:20000;IF 1:50-200
CompositionLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Purification ProcessThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity
Storage Condition-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized peptide derived from the Internal region of human POLR3G. AA range:151-200
Sequence
SpecificityPOLR3G Polyclonal Antibody detects endogenous levels of POLR3G protein.
Gene NamePOLR3G
Protein NameDNA-directed RNA polymerase III subunit RPC7
AliasPOLR3G;DNA-directed RNA polymerase III subunit RPC7;RNA polymerase III subunit C7;DNA-directed RNA polymerase III subunit G;RNA polymerase III 32 kDa subunit;RPC32
Organism-1Human
Gene ID-110622
SwissProt-1O15318
Organism-2Mouse
Gene ID-267486
SwissProt-2Q6NXY9
Organism-3
Gene ID-3
SwissProt-3
Organism-4
Gene ID-4
SwissProt-4
Cellular LocalizationNucleus . Cytoplasm . Excluded from nucleoli (PubMed:21898682). In zygotes and the 2-cell stage embryos, mainly in the cytoplasm. Starts to localize to the nucleus in the 8-16 cell stage embryo and early blastocysts (By similarity). .
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