PP2A α and β mouse mAb
Ucallm_WM0598-50
Price range: $268.00 through $328.00
Product details
Background:
This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes a beta isoform of the catalytic subunit. [provided by RefSeq, Mar 2010],
Specifications
| Size | 50μL/100μL |
| Target | PP2A α and β |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IP |
| MW(Calculated) | |
| MW(Observed) | 36kD |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:2000 |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 4C9 |
| Immunogen | Purified recombinant human full length PP2A beta protein expressed in E.coli |
| Specificity | This antibody detects endogenous levels of PP2A alpha and PP2A beta. |
| Gene Name | ppp2cb |
| Protein Name | |
| Other Name | PP2A beta; / PP2A-beta; / PP2AB_HUMAN; / PP2Abeta; / PP2CB; / Ppp2cb; / Protein phosphatase 2; / formerly 2A; / , catalytic subunit, beta isoform; / Protein phosphatase 2 catalytic subunit beta isozyme; / Protein phosphatase 2, catalytic subunit, beta isoform; / Protein phosphatase 2A catalytic subunit beta isoform; / Protein phosphatase type 2A catalytic subunit; / Serine/threonine protein phosphatase 2A catalytic subunit beta; / Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform. |
| Species | Human |
| Gene ID | 5516 |
| Uniprot | P62714, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | P62715 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm . Nucleus . Chromosome, centromere . Cytoplasm, cytoskeleton, spindle pole . In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. |
| Tissue Expression | Fibroblast,Heart,Kidney,Liver, |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Autophagy - animal;Cellular Processes >> Cell growth and death >> Cell cycle;Cellular Processes >> Cellular community - eukaryotes >> Tight junction;Organismal Systems >> Immune system >> T cell receptor signaling pathway;Organismal Systems >> Circulatory system >> Adrenergic signaling in cardiomyocytes;Organismal Systems >> Nervous system >> Dopaminergic synapse;Environmental Information Processing >> Signal transduction >> TGF-beta signaling pathway;Environmental Information Processing >> Signal transduction >> Hippo signaling pathway;Environmental Information Processing >> Signal transduction >> Sphingolipid signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway;Environmental Information Processing >> Signal transduction >> AMPK signaling pathway |
| Research Areas | >>mRNA surveillance pathway; / >>Sphingolipid signaling pathway; / >>Oocyte meiosis; / >>Autophagy - other; / >>Autophagy - animal; / >>PI3K-Akt signaling pathway; / >>AMPK signaling pathway; / >>Adrenergic signaling in cardiomyocytes; / >>TGF-beta signaling pathway; / >>Hippo signaling pathway; / >>Tight junction; / >>Dopaminergic synapse; / >>Long-term depression; / >>Chagas disease; / >>Hepatitis C; / >>Human papillomavirus infection |
| Function | Catalytic activity:A phosphoprotein + H(2)O = a protein + phosphate.,cofactor:Binds 1 iron ion per subunit.,cofactor:Binds 1 manganese ion per subunit.,Function:PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase.,PTM:Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.,PTM:Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle. Demethylated by PME1 (in vitro).,similarity:Belongs to the PPP phosphatase family.,similarity:Belongs to the PPP phosphatase family. PP-1 subfamily.,subcellular location:In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles.,subunit:PP2A consists of a common heterodimeric core enzyme (composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65) (subunit A)) that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Binds PPME1. May indirectly interact with SGOL1, most probably through regulatory B56 subunits., |
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