Specifications
| Size | 50μL/100μL |
| Target | NCSTN |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IF, IP, ELISA |
| MW(Calculated) | 78kD |
| MW(Observed) | 110-120kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1284R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | NCSTN KIAA0253 UNQ1874/PRO4317 |
| Protein Name | Nicastrin |
| Other Name | |
| Species | Human |
| Gene ID | 23385 |
| Uniprot | Q92542, |
| Species.1 | Mouse |
| Gene ID.1 | 59287 |
| Uniprot.1 | P57716, |
| Species.2 | Rat |
| Gene ID.2 | 289231 |
| Uniprot.2 | Q8CGU6 |
| Cellular Localization | Membrane; Single-pass type I membrane protein . Cytoplasmic vesicle membrane; Single-pass type I membrane protein . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
| Tissue Expression | Detected in brain (at protein level) (PubMed:10993067). Widely expressed (PubMed:11396676). |
| Signaling_pathway | Human Diseases >> Neurodegenerative disease >> Alzheimer disease;Environmental Information Processing >> Signal transduction >> Notch signaling pathway |
| Research Areas | |
| Function | Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein) . The gamma-secretase complex plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels. |
