U-Blot® RIP3 Rabbit mAb
WR4323 J774A.1 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with primary antibody(1:1000). The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
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Product details
Background:The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | RIP3 |
| Reactivity | Mouse |
| Applications | WB, IF, IP, ELISA |
| MW(Calculated) | 50kD |
| MW(Observed) | 50kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0528R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | RIPK3 RIP3 |
| Protein Name | Receptor-interacting serine/threonine-protein kinase 3 (EC 2.7.11.1) (RIP-like protein kinase 3) (Receptor-interacting protein 3) (RIP-3) |
| Other Name | Receptor-interacting serine/threonine-protein kinase 3; / RIP-like protein kinase 3; / Receptor-interacting protein 3; / RIP-3 |
| Species | Human |
| Gene ID | 11035 |
| Uniprot | Q9Y572, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q9QZL0 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm, Nucleus |
| Tissue Expression | Highly expressed in the pancreas. Detected at lower levels in heart, placenta, lung and kidney.; [Isoform 3]: Expression is significantly increased in colon and lung cancers. |
| Signaling_pathway | Cellular Processes >> Cell growth and death >> Necroptosis;Organismal Systems >> Immune system >> NOD-like receptor signaling pathway;Organismal Systems >> Immune system >> Cytosolic DNA-sensing pathway;Environmental Information Processing >> Signal transduction >> TNF signaling pathway |
| Research Areas | >>Necroptosis; / >>NOD-like receptor signaling pathway; / >>Cytosolic DNA-sensing pathway; / >>TNF signaling pathway; / >>Salmonella infection |
| Function | Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,Function:Promotes apoptosis.,PTM:Autophosphorylated.,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.,similarity:Contains 1 protein kinase domain.,subunit:Binds TRAF2 and RIPK1 and is recruited to the TNFR-1 signaling complex.,tissue specificity:Highly expressed in the pancreas. Detected at lower levels in heart, placenta, lung and kidney. Isoform 3 is significantly increased in colon and lung cancers., |
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