Name: U-Blot® SIRT1 Rabbit mAb
SKU: WR4074
Price: 268 USD
Availability: InStock

Product details

Background:This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008],

Specifications

Size	50μL/100μL
Target	SIRT1
Reactivity	Human, Rat
Applications	WB, IHC, IF, IP, ELISA
MW(Calculated)	81kD
MW(Observed)	130kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:100-200; WB 1:1000-5000; IF 1:200-1000; ELISA 1:5000-20000; IP 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0158R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	SIRT1
Protein Name	NAD-dependent protein deacetylase sirtuin-1
Other Name	SIRT1; / SIR2L1; / NAD-dependent protein deacetylase sirtuin-1; / hSIRT1; / Regulatory protein SIR2 homolog 1; / SIR2-like protein 1; / hSIR2
Species	Human
Gene ID	23411
Uniprot	Q96EB6,
Species.1	Mouse
Gene ID.1	93759
Uniprot.1	Q923E4
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Nuclear
Tissue Expression	Widely expressed.
Signaling_pathway	Cellular Processes >> Cell growth and death >> Cellular senescence;Organismal Systems >> Endocrine system >> Glucagon signaling pathway;Organismal Systems >> Aging >> Longevity regulating pathway;Organismal Systems >> Aging >> Longevity regulating pathway - multiple species;Human Diseases >> Cancer: overview >> MicroRNAs in cancer;Environmental Information Processing >> Signal transduction >> Wnt signaling pathway;Environmental Information Processing >> Signal transduction >> FoxO signaling pathway;Environmental Information Processing >> Signal transduction >> AMPK signaling pathway
Research Areas	>>Nicotinate and nicotinamide metabolism; / >>Metabolic pathways; / >>FoxO signaling pathway; / >>AMPK signaling pathway; / >>Longevity regulating pathway; / >>Longevity regulating pathway - multiple species; / >>Cellular senescence; / >>Glucagon signaling pathway; / >>Alcoholic liver disease; / >>Amphetamine addiction; / >>MicroRNAs in cancer
Function	Catalytic activity:NAD(+) + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein.,cofactor:Binds 1 zinc ion per subunit.,enzyme regulation:Inhibited by nicotinamide. Activated by resveratrol (3,5,4'-trihydroxy-trans-stilbene), butein (3,4,2',4'-tetrahydroxychalcone), piceatannol (3,5,3',4'-tetrahydroxy-trans-stilbene), Isoliquiritigenin (4,2',4'-trihydroxychalcone), fisetin (3,7,3',4'-tetrahydroxyflavone) and quercetin (3,5,7,3',4'-pentahydroxyflavone). RPS19BP1/AROS acts as a positive regulator of deacetylation activity.,Function:NAD-dependent deacetylase, which regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. Deacetylates 'Lys-382' of p53/TP53 and impairs its ability to induce proapoptotic program and modulate cell senescence. Deacetylates TAF1B and thereby represses rDNA transcription by the RNA polymerase I. Involved in HES1- and HEY2-mediated transcriptional repression. Inhibits skeletal muscle differentiation by deacetylating PCAF and MYOD1. May serve as a sensor of the cytosolic ratio of NAD(+)/NADH, which is essential in skeletal muscle cell differentiation. Despite some ability to deacetylate histones in vitro, such activity is either weak or inexistent in vivo. In case of HIV-1 infection, interacts with and deacetylates the viral Tat protein.,miscellaneous:Red wine, which contains resveratrol, may participate in activation of sirtuin proteins, and may therefore participate in an extended lifespan as it has been observed in yeast.,similarity:Belongs to the sirtuin family.,similarity:Contains 1 deacetylase sirtuin-type domain.,subcellular location:Recruited to the nuclear bodies via its interaction with PML.,subunit:Interacts with TAF1B. Found in a complex with PCAF and MYOD1 (By similarity). Interacts with MLLT7/FOXO4, HES1, HEY2, p53/TP53 and PML. Interacts with RPS19BP1/AROS.,tissue specificity:Widely expressed.,

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U-Blot® SIRT1 Rabbit mAb

Product details

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U-Blot® SIRT1 Rabbit mAb

Product details

Specifications

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