Price range: $268.00 through $328.00
Background:
The protein encoded by this gene is a membrane protein that functions to transport sodium and bicarbonate ions across the cell membrane. The encoded protein is important for pH regulation in neurons. The activity of this protein can be inhibited by 4, 4'-Di-isothiocyanatostilbene-2, 2'-disulfonic acid (DIDS). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012],
| Target | SLC4A8/10 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 140kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Modification | |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Source | |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Purity | |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human SLC4A8/10. AA range:411-460 |
| Sequence | |
| Specificity | SLC4A8/10 Polyclonal Antibody detects endogenous levels of SLC4A8/10 protein. |
| Gene Name | SLC4A8/SLC4A10 |
| Protein Name | Electroneutral sodium bicarbonate exchanger 1/Sodium-driven chloride bicarbonate exchanger |
| Other Name | SLC4A8; / KIAA0739; / NBC; / NBC3; / NDCBE1; / Electroneutral sodium bicarbonate exchanger 1; / Electroneutral Na; / +; / -driven Cl-HCO3 exchanger; / Solute carrier family 4 member 8; / k-NBC3; / SLC4A10; / NCBE; / Sodium-driven chloride bicarbonate exchanger; / Solute |
| Species | Human |
| Gene ID-1 | 57282;9498 |
| Uniprot | Q2Y0W8;Q6U841, |
| Species.1 | Mouse |
| Gene ID-2 | 59033;94229, |
| Uniprot.1 | |
| Species.2 | Rat |
| Gene ID-3 | 315311;295645 |
| Uniprot.2 | Q6RVG2;Q80ZA5 |
| Organism-4 | |
| Gene ID-4 | |
| SwissProt-4 | |
| Background | The protein encoded by this gene is a membrane protein that functions to transport sodium and bicarbonate ions across the cell membrane. The encoded protein is important for pH regulation in neurons. The activity of this protein can be inhibited by 4,4' -Di-isothiocyanatostilbene-2,2' -disulfonic acid (DIDS). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012], |
| Cellular Localization | Membrane; Multi-pass membrane protein . |
| Tissue Expression | Expressed in the pyramidal cells of the hippocampus (at protein level). Highly expressed in all major regions of the brain, spinal column and in testis, and moderate levels in trachea, thyroid and medulla region of kidney. Low expression levels observed in pancreas and kidney cortex. |
| Signaling_pathway | |
| Research Areas | |
| Function | Function:Mediates electroneutral sodium- and carbonate-dependent choride-HCO3(-) exchange with a Na(+):HCO3(-) stoichiometry of 2:1. Plays a major role in pH regulation in neurons. May be involved in cell pH regulation by transporting HCO3(-) from blood to cell. Enhanced expression in severe acid stress could be important for cell survival by mediating the influx of HCO3(-) into the cells. Also mediates lithium-dependent HCO3(-) cotransport. May be regulated by osmolarity.,miscellaneous:Activity is inhibited by 4,4'-Di-isothiocyanatostilbene-2,2'-disulfonic acid (DIDS - an inhibitor of several anionic channels and transporters).,similarity:Belongs to the anion exchanger (TC 2.A.31) family.,tissue specificity:Expressed in the pyramidal cells of the hippocampus (at protein level). Highly expressed in all major regions of the brain, spinal column and in testis, and moderate levels in trachea, thyroid and medulla region of kidney. Low expression levels observed in pancreas and kidney cortex., |
| RRID |
