SPOP Polyclonal Antibody
Ucallm_WP15765-50
Price range: $268.00 through $328.00
Product details
Background:
This gene encodes a protein that may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the encoded protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of this gene results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | SPOP |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| MW(Calculated) | |
| MW(Observed) | 42kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-2000; IHC 1:50-300 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human SPOP. AA range:41-90 |
| Specificity | SPOP Polyclonal Antibody detects endogenous levels of SPOP |
| Gene Name | SPOP |
| Protein Name | SPOP |
| Other Name | Speckle-type POZ protein; / HIB homolog 1; / Roadkill homolog 1 |
| Species | Human |
| Gene ID | 8405 |
| Uniprot | O43791, |
| Species.1 | Mouse |
| Gene ID.1 | 20747 |
| Uniprot.1 | Q6ZWS8 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus . Nucleus speckle . |
| Tissue Expression | Widely expressed. |
| Signaling_pathway | Environmental Information Processing >> Signal transduction >> Hedgehog signaling pathway |
| Research Areas | >>Hedgehog signaling pathway |
| Function | Domain:The MATH domain interacts with H2AFY and BMI1.,Function:Inhibits IPF1/PDX1 transactivation of established target promoters, such as insulin, may be by recruiting a repressor complex (By similarity). In complex with CUL3, involved in ubiquitination of BMI1, H2AFY and DAXX, and probably also in ubiquitination and proteasomal degradation of Gli2 or Gli3.,miscellaneous:Antigen recognized by serum from scleroderma patient.,pathway:Protein modification; protein ubiquitination.,similarity:Belongs to the Tdpoz family.,similarity:Contains 1 BTB (POZ) domain.,similarity:Contains 1 MATH domain.,subunit:Homodimer. Part of a complex consisting of BMI1, CUL3 and SPOP. Part of a complex consisting of H2AFY, CUL3 and SPOP. Part of a complex consisting of DAXX, CUL3 and SPOP. Interacts with H2AFY, IPF1/PDX1, BMI1 and DAXX. Interacts with CUL3.,tissue specificity:Widely expressed., |
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