TRAIL Polyclonal Antibody
Ucallm_WP14841-50
Price range: $268.00 through $328.00
Product details
Background:
The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. This protein binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provi
Specifications
| Size | 50μL/100μL |
| Target | TRAIL |
| Reactivity | Human, Mouse |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 30kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human TNFSF10. AA range:31-80 |
| Specificity | TRAIL Polyclonal Antibody detects endogenous levels of TRAIL protein. |
| Gene Name | TNFSF10 |
| Protein Name | Tumor necrosis factor ligand superfamily member 10 |
| Other Name | TNFSF10; / APO2L; / TRAIL; / Tumor necrosis factor ligand superfamily member 10; / Apo-2 ligand; / Apo-2L; / TNF-related apoptosis-inducing ligand; / Protein TRAIL; / CD antigen CD253 |
| Species | Human |
| Gene ID | 8743 |
| Uniprot | P50591, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | P50592 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cell membrane; Single-pass type II membrane protein . Secreted . Exists both as membrane-bound and soluble form. . |
| Tissue Expression | Widespread; most predominant in spleen, lung and prostate. |
| Signaling_pathway | Cellular Processes >> Cell growth and death >> Apoptosis;Cellular Processes >> Cell growth and death >> Necroptosis;Organismal Systems >> Immune system >> Natural killer cell mediated cytotoxicity;Environmental Information Processing >> Signal transduction >> FoxO signaling pathway;Environmental Information Processing >> Signaling molecules and interaction >> Cytokine-cytokine receptor interaction;Environmental Information Processing >> Signaling molecules and interaction >> Viral protein interaction with cytokine and cytokine receptor |
| Research Areas | >>Cytokine-cytokine receptor interaction; / >>Viral protein interaction with cytokine and cytokine receptor; / >>FoxO signaling pathway; / >>Apoptosis; / >>Necroptosis; / >>Natural killer cell mediated cytotoxicity; / >>Pathogenic Escherichia coli infection; / >>Salmonella infection; / >>Influenza A; / >>Lipid and atherosclerosis |
| Function | cofactor:Binds 1 zinc ion per trimer.,Function:Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.,similarity:Belongs to the tumor necrosis factor family.,subunit:Homotrimer.,tissue specificity:Widespread; most predominant in spleen, lung and prostate., |
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