U-Blot® Acetylated-Histone H3 ELISA Kit
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$999.00
Product details
The Acetylated-Histone H3 ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human and mouse cell lysates. By determining Acetylated Histone H3 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human and mouse Acetylated-Histone H3. An anti-pan Histone H3 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-Acetylated-Histone H3 antibody is used to detect Acetylated Histone H3. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Acetylated Histone H3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.This assay platform allows rapid quantification of acetylated protein in various cell lysates and supports high-throughput screening of a large number of different cell lysate samples without the time-consuming and labor-intensive Western Blot analysis. It features minimal hands-on operation time, user-friendly workflow and a fully non-radioactive detection system, ensuring safe, efficient and convenient experimental operation.
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human,Mouse |
| Compatible Sample Types | Tissue Lysates, Cell Lysates |
| Accession Number | P84243| P84244 |
| Gene Id | 3020|3021 |
| Gene Symbols | H3F3A,H3F3B,H3.3A,H3F3,PP781,H3.3B |
| Protein Name / Synonyms | Histone H3.3 |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Post-Translational Modifications, Acetylation, Epigenetics |
| Modification | |
| Shipping Type | 2‑8 ℃ |
| Storage | -20°C |
| Kit Components | |
| Additional Materials Required | |
| Assay Procedure Summary | |
| Storage/Stability | Upon receipt, the kit should be stored at -20°C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4°C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge, and store at -20°C. Item D, store at 2-8°C for up to one month (store at -20°C for up to 6 months, avoid repeated freeze-thaw cycles). Reconstituted Positive Control (Item K) should be stored at -70°C. |
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