U-Blot® Acetylated PALM and Total PALM ELISA Kit
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$999.00
Product details
The Acetylated-PALM and Total-PALM ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human acetylated ATP-citrate synthase and total ATP-citrate synthase. An anti-pan PALM antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and PALM present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-acetylation antibody is used to detect acetylated PALM or biotinylated anti-PALM antibody is used to detect total PALM . After washing away unbound antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of acetylated PALM or total PALM bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.This assay enables quick and easy quantification of target proteins and allows high-throughput screening of numerous different lysates without the need for traditional Western Blot analysis. It features minimal hands-on operation time, simple workflow, and completely non-radioactive detection system, ensuring safe and convenient experimental operation.
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Compatible Sample Types | Tissue Lysates, Cell Lysates |
| Accession Number | O75781 |
| Gene Id | 5064 |
| Gene Symbols | PALM, KIAA0270 |
| Protein Name / Synonyms | Paralemmin-1; Paralemmin |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Post-Translational Modifications, Acetylation, Epigenetics |
| Modification | Acetylation |
| Shipping Type | 2‑8 ℃ |
| Storage | -20°C |
| Kit Components | Microplate Coated With Anti-Pan-PALM Antibody:1 plate;Anti-Acetylation Detection Antibody:1 vial;Anti-PALM Detection Antibody:1 vial;HRP-Conjugated Streptavidin:200 µL;Wash Buffer:25 mL;Assay Diluent:15 mL;Lysis Buffer:5 mL;TMB One-Step Substrate Reagent:12 mL;Stop Solution:8 mL; |
| Additional Materials Required | Microplate reader capable of measuring absorbance at 450 nm;Protease and Phosphatase inhibitors;Precision pipettes to deliver 2 µL to 1 mL volumes;Adjustable 1-25 mL pipettes for reagent preparation;100 mL and 1 liter graduated cylinders;Log-log graph paper or computer and software for ELISA data analysis;Absorbent paper;Tubes to prepare sample dilutions;Distilled or deionized water;Shaker |
| Assay Procedure Summary | Prepare all reagents and samples as instructed; Add 100 µL sample to each well. Incubate 2.5 hours at room temperature or overnight at 4°C with gentle shaking; Add 100 µL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking; Add 100 µL prepared HRP-Conjugated Streptavidin solution. Incubate for 1 hour at room temperature with gentle shaking; Add 100 µL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature; Add 50 µL Stop Solution to each well. Read at 450 nm immediately. |
| Storage/Stability | The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles. |
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