U-Blot® ACSL1 Rabbit mAb
Ucallm_WR5240-50
Price range: $268.00 through $328.00
Product details
Background:The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013],
Specifications
| Size | 50μL/100μL |
| Target | ACSL1 |
| Reactivity | Human |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 78kD |
| MW(Observed) | 78kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1477R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | ACSL1 FACL1 FACL2 LACS LACS1 LACS2 |
| Protein Name | Long-chain-fatty-acid--CoA ligase 1 (Acyl-CoA synthetase 1) (ACS1) (Long-chain acyl-CoA synthetase 1) (LACS 1) (Long-chain acyl-CoA synthetase 2) (LACS 2) (Long-chain fatty acid-CoA ligase 2) (Palmitoyl-CoA ligase 1) (Palmitoyl-CoA ligase 2) |
| Other Name | |
| Species | Human |
| Gene ID | 2180 |
| Uniprot | P33121, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | P41216, |
| Species.2 | Rat |
| Gene ID.2 | |
| Uniprot.2 | P18163 |
| Cellular Localization | Mitochondrion outer membrane; Single-pass type III membrane protein . Peroxisome membrane; Single-pass type III membrane protein . Microsome membrane; Single-pass type III membrane protein . Endoplasmic reticulum membrane; Single-pass type III membrane protein . |
| Tissue Expression | Highly expressed in liver, heart, skeletal muscle, kidney and erythroid cells, and to a lesser extent in brain, lung, placenta and pancreas. |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Peroxisome;Cellular Processes >> Cell growth and death >> Ferroptosis;Organismal Systems >> Endocrine system >> Adipocytokine signaling pathway;Organismal Systems >> Endocrine system >> PPAR signaling pathway |
| Research Areas | >>Fatty acid biosynthesis; / >>Fatty acid degradation; / >>Metabolic pathways; / >>Fatty acid metabolism; / >>PPAR signaling pathway; / >>Peroxisome; / >>Ferroptosis; / >>Thermogenesis; / >>Adipocytokine signaling pathway |
| Function | Catalytic activity:ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA.,cofactor:Magnesium.,developmental stage:Expressed during the early stages of erythroid development while expression is very low in reticulocytes and young erythrocytes.,Function:Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitoleate, oleate and linoleate.,similarity:Belongs to the ATP-dependent AMP-binding enzyme family.,tissue specificity:Highly expressed in liver, heart, skeletal muscle, kidney and erythroid cells, and to a lesser extent in brain, lung, placenta and pancreas., |
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