U-Blot® ACTN4 Rabbit mAb
Ucallm_WR5948-50
Price range: $268.00 through $328.00
Product details
Background：
Specifications
| Size | 50μL/100μL |
| Target | ACTN4 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 105kD |
| MW(Observed) | 105kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1920R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | ACTN4 |
| Protein Name | Alpha-actinin-4 (F-actin cross-linking protein) (Non-muscle alpha-actinin 4) |
| Other Name | |
| Species | Human |
| Gene ID | 81 |
| Uniprot | O43707, |
| Species.1 | Mouse |
| Gene ID.1 | 60595 |
| Uniprot.1 | P57780, |
| Species.2 | Rat |
| Gene ID.2 | 63836 |
| Uniprot.2 | Q9QXQ0 |
| Cellular Localization | Nucleus . Cytoplasm . Cell junction . Cytoplasm, cytoskeleton, stress fiber . Cytoplasm, perinuclear region . Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Expressed in the perinuclear rim and manchette structure in early elongating spermatids during spermiogenesis (By similarity). Nuclear translocation can be induced by the PI3 kinase inhibitor wortmannin or by cytochalasin D. Exclusively localized in the nucleus in a limited number of cell lines (breast cancer cell line MCF-7, oral floor cancer IMC-2, and bladder cancer KU-7). . |
| Tissue Expression | Widely expressed. |
| Signaling_pathway | Cellular Processes >> Cellular community - eukaryotes >> Focal adhesion;Cellular Processes >> Cellular community - eukaryotes >> Adherens junction;Cellular Processes >> Cellular community - eukaryotes >> Tight junction;Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Organismal Systems >> Immune system >> Leukocyte transendothelial migration;Human Diseases >> Immune disease >> Systemic lupus erythematosus |
| Research Areas | |
| Function | F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation . Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions (By similarity). May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA . |
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