U-Blot® ALOX12 Rabbit mAb
Ucallm_WR5980-50
Price range: $268.00 through $328.00
Product details
Background：
Specifications
| Size | 50μL/100μL |
| Target | ALOX12 |
| Reactivity | Human, Rat |
| Applications | WB, IF, ELISA |
| MW(Calculated) | 76kD |
| MW(Observed) | 70kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM2045R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | ALOX12 LOG12 |
| Protein Name | Arachidonate 12-lipoxygenase, 12S-type (12S-LOX) (12S-lipoxygenase) (Platelet-type lipoxygenase 12) |
| Other Name | Arachidonate 12-lipoxygenase, 12S-type; / 12S-LOX; / 12S-lipoxygenase; / Platelet-type lipoxygenase 12 |
| Species | Human |
| Gene ID | 239 |
| Uniprot | P18054, |
| Species.1 | Mouse |
| Gene ID.1 | 11684 |
| Uniprot.1 | P39655 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm, cytosol. Membrane. Membrane association is stimulated by EGF. |
| Tissue Expression | Expressed in vascular smooth muscle cells. |
| Signaling_pathway | Organismal Systems >> Sensory system >> Inflammatory mediator regulation of TRP channels |
| Research Areas | |
| Function | Catalyzes the regio and stereo-specific incorporation of molecular oxygen into free and esterified polyunsaturated fatty acids generating lipid hydroperoxides that can be further reduced to the corresponding hydroxy species . Mainly converts arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) to the specific bioactive lipid (12S)-hydroperoxyeicosatetraenoate/(12S)-HPETE . Through the production of bioactive lipids like (12S)-HPETE it regulates different biological processes including platelet activation . It can also catalyze the epoxidation of double bonds of polyunsaturated fatty acids such as (14S)-hydroperoxy-docosahexaenoate/(14S)-HPDHA resulting in the formation of (13S,14S)-epoxy-DHA . Furthermore, it may participate in the sequential oxidations of DHA ((4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate) to generate specialized pro-resolving mediators (SPMs) like resolvin D5 ((7S,17S)-diHPDHA) and (7S,14S)-diHPDHA, that actively down-regulate the immune response and have anti-aggregation properties with platelets . An additional function involves a multistep process by which it transforms leukotriene A4/LTA4 into the bioactive lipids lipoxin A4/LXA4 and lipoxin B4/LXB4, both are vasoactive and LXA4 may regulate neutrophil function via occupancy of specific recognition sites . Can also peroxidize linoleate ((9Z,12Z)-octadecadienoate) to (13S)-hydroperoxyoctadecadienoate/ (13S-HPODE) (By similarity). Due to its role in regulating both the expression of the vascular endothelial growth factor (VEGF, an angiogenic factor involved in the survival and metastasis of solid tumors) and the expression of integrin beta-1 (known to affect tumor cell migration and proliferation), it can be regarded as protumorigenic . Important for cell survival, as it may play a role not only in proliferation but also in the prevention of apoptosis in vascular smooth muscle cells . |
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