U-Blot® Arginase II Rabbit mAb
Ucallm_WR6022-50
Price range: $268.00 through $328.00
Product details
Background：
Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exists (types I and II) which differ in their tissue distribution , subcellular localization , immunologic crossreactivity and physiologic function. The type II isoform encoded by this gene , is located in the mitochondria and expressed in extra-hepatic tissues , especially kidney. The physiologic role of this isoform is poorly understood; it is thought to play a role in nitric oxide and polyamine metabolism. Transcript variants of the type II gene resulting from the use of alternative polyadenylation sites have been described. [provided by RefSeq , Jul 2008] ,
Specifications
| Size | 50μL/100μL |
| Target | Arginase II |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 39kD |
| MW(Observed) | 40kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM2013R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | ARG2 |
| Protein Name | Arginase-2 mitochondrial |
| Other Name | ARG2; / Arginase-2; / mitochondrial; / Kidney-type arginase; / Non-hepatic arginase; / Type II arginase |
| Species | Human |
| Gene ID | 384 |
| Uniprot | P78540, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | O08691 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Mitochondrion . |
| Tissue Expression | Expressed most strongly in kidney and prostate, much less strongly in the brain, skeletal muscle, placenta, lung, mammary gland, macrophage, uterus, testis and gut, but apparently not in the liver, heart and pancreas. Expressed in activated T cells (PubMed:27745970). |
| Signaling_pathway | |
| Research Areas | >>Arginine biosynthesis; / >>Arginine and proline metabolism; / >>Metabolic pathways; / >>Biosynthesis of amino acids; / >>Amoebiasis |
| Function | Catalytic activity:L-arginine + H(2)O = L-ornithine + urea.,cofactor:Binds 2 manganese ions per subunit.,Function:May play a role in the regulation of extra-urea cycle arginine metabolism and also in down-regulation of nitric oxide synthesis. Extrahepatic arginase functions to regulate L-arginine bioavailability to NO synthase. Since NO synthase is found in the penile corpus cavernosum smooth muscle, the clitoral corpus cavernosum and the vagina, arginase II plays a role in both male and female sexual arousal. It is therefore a potential target for the treatment of male and female sexual arousal disorders.,online information:Arginase entry,pathway:Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.,similarity:Belongs to the arginase family.,subunit:Homotrimer.,tissue specificity:Expressed most strongly in kidney and prostate, much less strongly in the brain, skeletal muscle, placenta, lung, mammary gland, macrophage, uterus, testis and gut, but apparently not in the liver, heart and pancreas., |
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