U-Blot® GAPDH Mouse mAb
Ucallm_WM1871-50
Price range: $268.00 through $328.00
Product details
Background：
Specifications
| Size | 50μL/100μL |
| Target | GAPDH |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 38kD |
| MW(Observed) | 38kD |
| Host Species | Mouse |
| Isotype | IgG2b, kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:1000-1:4000; WB 1:10000-1:50000; IF 1:100-500; ELISA 1:1000-5000; IP 1:50-1:200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein G affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RMMR1416 |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | GAPDH |
| Protein Name | Glyceraldehyde-3-phosphate dehydrogenase |
| Other Name | GAPDH; / GAPD; / CDABP0047; / OK/SW-cl.12; / Glyceraldehyde-3-phosphate dehydrogenase; / GAPDH; / Peptidyl-cysteine S-nitrosylase GAPDH |
| Species | Human |
| Gene ID | 2597 |
| Uniprot | P04406, |
| Species.1 | Mouse |
| Gene ID.1 | 100042025 |
| Uniprot.1 | P16858, |
| Species.2 | Rat |
| Gene ID.2 | 24383 |
| Uniprot.2 | P04797 |
| Cellular Localization | Nucleus,Cytoplasmic,Cytoplasm, cytoskeleton, microtubule organizing center, centrosome ,Peroxisome matrix, |
| Tissue Expression | Astrocytoma,Brain,Cajal-Retzius cell,Colon adenocarcinoma,Epitheliu |
| Signaling_pathway | Human Diseases >> Neurodegenerative disease >> Alzheimer disease;Environmental Information Processing >> Signal transduction >> HIF-1 signaling pathway |
| Research Areas | >>Glycolysis / Gluconeogenesis; / >>Metabolic pathways; / >>Carbon metabolism; / >>Biosynthesis of amino acids; / >>HIF-1 signaling pathway; / >>Alzheimer disease; / >>Pathogenic Escherichia coli infection; / >>Salmonella infection; / >>Diabetic cardiomyopathy |
| Function | Catalytic activity:D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH.,Function:Independent of its glycolytic activity it is also involved in membrane trafficking in the early secretory pathway.,online information:Glyceraldehyde 3-phosphate dehydrogenase entry,pathway:Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1.,pathway:Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.,PTM:Reversible S-nitrosylation of Cys-152 inhibits enzymatic activity and increases endogenous ADP-ribosylation, which inhibits the enzyme in a non-reversible manner. The latter modification is more likely to be a pathophysiological event associated with inhibition of gluconeogenesis.,sequence Caution:Differs quite extensively.,similarity:Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.,subcellular location:Postnuclear and Perinuclear regions.,subunit:Homotetramer.,subunit:Homotetramer. Binds PRKCI., |
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