Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Compatible Sample Types | Tissue Lysates, Cell Lysates |
| Accession Number | P37840 |
| Gene Id | 6622 |
| Gene Symbols | SNCA NACP PARK1 |
| Protein Name / Synonyms | Alpha-synuclein (Non-A beta component of AD amyloid) (Non-A4 component of amyloid precursor) (NACP) |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Neuroscience, Post-Translational Modifications, Inflammation, Autophagy, Nitration, Parkinson's Disease, Aging |
| Modification | |
| Shipping Type | 2‑8 ℃ |
| Storage | -20°C |
| Kit Components | |
| Additional Materials Required | Microplate reader capable of measuring absorbance at 450 nm;Protease and Phosphatase inhibitors;Precision pipettes to deliver 2 µL to 1 mL volumes;Adjustable 1-25 mL pipettes for reagent preparation;100 mL and 1 liter graduated cylinders;Log-log graph paper or computer and software for ELISA data analysis;Absorbent paper;Tubes to prepare positive control or sample dilutions;Distilled or deionized water |
| Assay Procedure Summary | Prepare all reagents, samples, and Positive Control as instructed. in the manual; Add 100 µL Standard to each well; Incubate 2.5 hours at room temperature or overnight at 4ºC with gentle shaking; Add 100 µL prepared detection antibody to each well; Incubate for 1 hour at room temperature with gentle shaking; Add 100 µL prepared HRP-conjugated anti-rabbit IgG concentrate solution; Incubate for 1 hour at room temperature with gentle shaking; Add 100 µL TMB One-Step Substrate Reagent to each well; Incubate 30 minutes at room temperature; Add 50 µL Stop Solution to each well; Read at 450 nm immediately. |
| Storage/Stability | The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles. |
