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Background:The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis. [provided by RefSeq, Jul 2008],
| Target | MARCKS |
| Reactivity | Human |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 31kD |
| MW(Observed) | 80kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Modification | |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Source | |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Purity | |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | PT1586R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Sequence | |
| Specificity | Endogenous |
| Gene Name | MARCKS |
| Protein Name | Myristoylated alanine-rich C-kinase substrate |
| Other Name | MARCKS; / MACS; / PRKCSL; / Myristoylated alanine-rich C-kinase substrate; / MARCKS; / Protein kinase C substrate; / 80 kDa protein, light chain; / 80K-L protein; / PKCSL |
| Species | Human |
| Gene ID-1 | 4082 |
| Uniprot | P29966, |
| Species.1 | Mouse |
| Gene ID-2 | 17118 |
| Uniprot.1 | P26645, |
| Species.2 | Rat |
| Gene ID-3 | |
| Uniprot.2 | P30009 |
| Organism-4 | |
| Gene ID-4 | |
| SwissProt-4 | |
| Background | The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis. [provided by RefSeq, Jul 2008], |
| Cellular Localization | Cytoplasm, cytoskeleton . Membrane; Lipid-anchor . |
| Tissue Expression | Blood,Brain,Epithelium,Muscle,Skin, |
| Signaling_pathway | Organismal Systems >> Immune system >> Fc gamma R-mediated phagocytosis;Human Diseases >> Cancer: overview >> MicroRNAs in cancer |
| Research Areas | >>Fc gamma R-mediated phagocytosis; / >>MicroRNAs in cancer |
| Function | Function:MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.,PTM:Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity.,similarity:Belongs to the MARCKS family., |
| RRID |
