Name: U-Blot® Neurofilament(NF-L) Mouse mAb (Ready to Use)
SKU: WM1722R
Price: 168 USD
Availability: InStock

Product details

Background：
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008],

Specifications

Target	NF-L
Reactivity	Human
Applications	IHC
MW(Calculated)
MW(Observed)
Host Species	Mouse
Isotype	IgG2b,Kappa
Conjugate/Modification	Unmodified
Modification
Recommended Dilution Ratio	Ready to use for IHC / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004)
Formulation	The prediluted ready-to-use antibody is diluted in phosphate buffer saline containing stabilizing protein and 0.05% Proclin 300
Source
Purification	The antibody was affinity-purified from ascites by affinity-chromatography using specific immunogen.
Purity
Storage	2°C to 8°C/1 year,Ship by ice bag
Concentration
Clonality	Monoclonal
Clone Number	ABT208
Immunogen	Synthesized peptide derived from human Neurofilament AA range: 400-543
Sequence
Specificity	The antibody can specifically recognize human Neurofilament protein, especilaly NF-L protein.
Gene Name	NEFL NF68 NFL
Protein Name	Neurofilament light polypeptide; NF-L; Neurofilament triplet L protein; 68 kDa neurofilament protein
Other Name	Neurofilament light polypeptide; / NF-L; / 68 kDa neurofilament protein; / Neurofilament triplet L protein
Species	Human
Gene ID-1	4747
Uniprot	P07196,
Species.1	Mouse
Gene ID-2	18039
Uniprot.1	P08551,
Species.2	Rat
Gene ID-3	83613
Uniprot.2	P19527
Organism-4
Gene ID-4
SwissProt-4
Background	Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008],
Cellular Localization	Cytoplasmic
Tissue Expression	Cytoplasmic
Signaling_pathway	Human Diseases >> Neurodegenerative disease >> Amyotrophic lateral sclerosis;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases
Research Areas	>>Amyotrophic lateral sclerosis; / >>Pathways of neurodegeneration - multiple diseases
Function	Caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,Disease:Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 1F (CMT1F) [MIM:607734]. CMT1F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. CMT1F is characterized by onset in infancy or childhood (range 1 to 13 years).,Disease:Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 2E (CMT2E) [MIM:607684]. CMT2E is an autosomal dominant form of Charcot-Marie-Tooth disease type 2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy.,Domain:The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions.,Function:Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.,miscellaneous:NF-L is the most abundant of the three neurofilament proteins and, as the other nonepithelial intermediate filament proteins, it can form homopolymeric 10-nm filaments.,PTM:O-glycosylated.,similarity:Belongs to the intermediate filament family.,subunit:Interacts with RGNEF.,
RRID

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U-Blot® Neurofilament(NF-L) Mouse mAb (Ready to Use)

Product details

Specifications

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U-Blot® Neurofilament(NF-L) Mouse mAb (Ready to Use)

Product details

Specifications

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