U-Blot® OGG1 Rabbit mAb
Ucallm_WR5325-50
Price range: $268.00 through $328.00
Product details
Background:This gene encodes the enzyme responsible for the excision of 8-oxoguanine, a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen. The action of this enzyme includes lyase activity for chain cleavage. Alternative splicing of the C-terminal region of this gene classifies splice variants into two major groups, type 1 and type 2, depending on the last exon of the sequence. Type 1 alternative splice variants end with exon 7 and type 2 end with exon 8. All variants share the N-terminal region in common, which contains a mitochondrial targeting signal that is essential for mitochondrial localization. Many alternative splice variants for this gene have been described, but the full-length nature for every variant has not been determined. [provided by RefSeq, Aug 2008],
Specifications
| Size | 50μL/100μL |
| Target | OGG1 |
| Reactivity | Human |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 39kD |
| MW(Observed) | 39kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:10000-1:50000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1557R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | OGG1 MMH MUTM OGH1 |
| Protein Name | N-glycosylase/DNA lyase [Includes: 8-oxoguanine DNA glycosylase lyase (AP lyase) (EC 4.2.99.18)] |
| Other Name | |
| Species | Human |
| Gene ID | 4968 |
| Uniprot | O15527, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | O08760, |
| Species.2 | Rat |
| Gene ID.2 | |
| Uniprot.2 | O70249 |
| Cellular Localization | Nucleus, nucleoplasm . Nucleus speckle . Nucleus matrix . Together with APEX1 is recruited to nuclear speckles in UVA-irradiated cells.; [Isoform 1A]: Nucleus.; [Isoform 2A]: Mitochondrion. |
| Tissue Expression | Ubiquitous. |
| Signaling_pathway | |
| Research Areas | >>Base excision repair |
| Function | Catalytic activity:The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.,Disease:Defects in OGG1 are a cause of renal cell carcinoma (RCC1) [MIM:144700].,Disease:Defects in OGG1 are associated with tumor formation.,Function:DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion.,similarity:Belongs to the type-1 OGG1 family.,tissue specificity:Ubiquitous., |
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