Specifications
| Size | 50μL/100μL |
| Target | OTUB1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 31kD |
| MW(Observed) | 31kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1734R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | OTUB1 |
| Protein Name | Ubiquitin thioesterase OTUB1 |
| Other Name | OTUB1; / OTB1; / OTU1; / HSPC263; / Ubiquitin thioesterase OTUB1; / Deubiquitinating enzyme OTUB1; / OTU domain-containing ubiquitin aldehyde-binding protein 1; / Otubain-1; / hOTU1; / Ubiquitin-specific-processing protease OTUB1 |
| Species | Human |
| Gene ID | 55611 |
| Uniprot | Q96FW1, |
| Species.1 | Mouse |
| Gene ID.1 | 107260 |
| Uniprot.1 | Q7TQI3, |
| Species.2 | Rat |
| Gene ID.2 | 293705 |
| Uniprot.2 | B2RYG6 |
| Cellular Localization | Cytoplasm . |
| Tissue Expression | Isoform 1 is ubiquitous. Isoform 2 is expressed only in lymphoid tissues such as tonsils, lymph nodes and spleen, as well as peripheral blood mononuclear cells. |
| Signaling_pathway | |
| Research Areas | |
| Function | Function:Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. Acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy. Isoform 1 destabilizes RNF128, leading to prevent anergy. In contrast, isoform 2 stabilizes RNF128 and promotes anergy. Surprisingly, it regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128.,similarity:Belongs to the peptidase C65 family.,similarity:Contains 1 OTU domain.,subunit:Isoform 1 and isoform 2 interact with RNF128. Isoform 1 forms a ternary complex with RNF128 and USP8. Isoform 1 interacts with the C-terminal UCH catalytic domain of USP8. Isoform 2 does not associate with USP8.,tissue specificity:Isoform 1 is ubiquitous. Isoform 2 is expressed only in lymphoid tissues such as tonsils, lymph nodes and spleen, as well as peripheral blood mononuclear cells., |
