Price range: $268.00 through $328.00
Background：
This gene encodes the liver (L) subunit of an enzyme that catalyzes the conversion of D-fructose 6-phosphate to D-fructose 1 ,6-bisphosphate , which is a key step in glucose metabolism (glycolysis) . This enzyme is a tetramer that may be composed of different subunits encoded by distinct genes in different tissues. Alternative splicing results in multiple transcript variants. [provided by RefSeq , Mar 2014] ,
| Size | 50μL/100μL |
| Target | PFK-B |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 85kD |
| MW(Observed) | 85kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1784R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | PFKL |
| Protein Name | 6-phosphofructokinase liver type |
| Other Name | PFKL; / 6-phosphofructokinase; / liver type; / Phosphofructo-1-kinase isozyme B; / PFK-B; / Phosphofructokinase 1; / Phosphohexokinase |
| Species | Human |
| Gene ID | 5211 |
| Uniprot | P17858, |
| Species.1 | Mouse |
| Gene ID.1 | 18641 |
| Uniprot.1 | P12382, |
| Species.2 | Rat |
| Gene ID.2 | 25741 |
| Uniprot.2 | P30835 |
| Cellular Localization | Cytoplasm . |
| Tissue Expression | Brain,Cervix,Colon,Human amygdala,Human retina,Kidney,Liver,Lung,Muscle,Pla |
| Signaling_pathway | Organismal Systems >> Endocrine system >> Glucagon signaling pathway;Organismal Systems >> Endocrine system >> Thyroid hormone signaling pathway;Human Diseases >> Cancer: overview >> Central carbon metabolism in cancer;Environmental Information Processing >> Signal transduction >> HIF-1 signaling pathway;Environmental Information Processing >> Signal transduction >> AMPK signaling pathway;Genetic Information Processing >> Folding, sorting and degradation >> RNA degradation |
| Research Areas | >>Glycolysis / Gluconeogenesis; / >>Pentose phosphate pathway; / >>Fructose and mannose metabolism; / >>Galactose metabolism; / >>Metabolic pathways; / >>Carbon metabolism; / >>Biosynthesis of amino acids; / >>RNA degradation; / >>HIF-1 signaling pathway; / >>AMPK signaling pathway; / >>Thyroid hormone signaling pathway; / >>Glucagon signaling pathway; / >>Central carbon metabolism in cancer |
| Function | Catalytic activity:ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.,cofactor:Magnesium.,enzyme regulation:Allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate.,miscellaneous:In human PFK exists as a system of 3 types of subunits, PFKM (muscle), PFKL (liver) and PFKP (platelet) isoenzymes.,pathway:Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.,similarity:Belongs to the phosphofructokinase family. Two domains subfamily.,subunit:Tetramer. Muscle is M4, liver is L4, and red cell is M3L, M2L2, or ML3., |
