U-Blot® RIP Rabbit mAb
Ucallm_WR6095-50
Price range: $268.00 through $328.00
Product details
Background:catalytic activity:ATP + a protein = ADP + a phosphoprotein. ,function:Promotes apoptosis and activation of NF-kappa-B. Required for TNFRSF1A mediated activation of NF-kappa-B. ,PTM:Autophosphorylated on serine and threonine residues. ,PTM:Proteolytically cleaved by caspase-8 during TNF-induced apoptosis. Cleavage abolishes NF-kappa-B activation and enhances pro-apototic signaling through the TRADD-FADD interaction. ,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. ,similarity:Contains 1 death domain. ,similarity:Contains 1 protein kinase domain. ,subunit:Binds to the death domain of TNFRSF6 and TRADD. Is recruited by TRADD to TNFRSF1A in a TNF-dependent process. Binds RIPK3 , UBCE7IP1 isoform 3 (ZIN) , EGFR , IKBKG , TRAF1 , TRAF2 and TRAF3. Interacts with BNLF1. Interacts with SQSTM1 upon TNF-alpha stimulation. May interacts with MAVS/IPS1. ,
Specifications
| Size | 50μL/100μL |
| Target | RIP |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 76kD |
| MW(Observed) | 76kD |
| Host Species | Rabbit |
| Isotype | IgG, Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year (Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM2062R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | RIPK1 RIP RIP1 |
| Protein Name | Receptor-interacting serine/threonine-protein kinase 1 (Cell death protein RIP) (Receptor-interacting protein 1) (RIP-1) (Serine/threonine-protein kinase RIP) |
| Other Name | |
| Species | Human |
| Gene ID | 8737 |
| Uniprot | Q13546, |
| Species.1 | Mouse |
| Gene ID.1 | 19766 |
| Uniprot.1 | Q60855 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasmic , Membranous |
| Tissue Expression | Leukemic T-cell ,T-cell ,Umbilical vein endothelial cell , |
| Signaling_pathway | Cellular Processes >> Cell growth and death >> Apoptosis;Cellular Processes >> Cell growth and death >> Necroptosis;Organismal Systems >> Immune system >> Toll-like receptor signaling pathway;Organismal Systems >> Immune system >> NOD-like receptor signaling pathway;Organismal Systems >> Immune system >> RIG-I-like receptor signaling pathway;Organismal Systems >> Immune system >> Cytosolic DNA-sensing pathway;Environmental Information Processing >> Signal transduction >> NF-kappa B signaling pathway;Environmental Information Processing >> Signal transduction >> TNF signaling pathway |
| Research Areas | >>NF-kappa B signaling pathway; / >>Apoptosis; / >>Necroptosis; / >>Toll-like receptor signaling pathway; / >>NOD-like receptor signaling pathway; / >>RIG-I-like receptor signaling pathway; / >>Cytosolic DNA-sensing pathway; / >>TNF signaling pathway; / >>Alcoholic liver disease; / >>Pathogenic Escherichia coli infection; / >>Shigellosis; / >>Salmonella infection; / >>Hepatitis C; / >>Human cytomegalovirus infection; / >>Epstein-Barr virus infection; / >>Human immunodeficiency virus 1 infection |
| Function | Catalytic activity:ATP + a protein = ADP + a phosphoprotein. ,Function:Promotes apoptosis and activation of NF-kappa-B. Required for TNFRSF1A mediated activation of NF-kappa-B. ,PTM:Autophosphorylated on serine and threonine residues. ,PTM:Proteolytically cleaved by caspase-8 during TNF-induced apoptosis. Cleavage abolishes NF-kappa-B activation and enhances pro-apototic signaling through the TRADD-FADD interaction. ,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. ,similarity:Contains 1 death domain. ,similarity:Contains 1 protein kinase domain. ,subunit:Binds to the death domain of TNFRSF6 and TRADD. Is recruited by TRADD to TNFRSF1A in a TNF-dependent process. Binds RIPK3 , UBCE7IP1 isoform 3 (ZIN) , EGFR , IKBKG , TRAF1 , TRAF2 and TRAF3. Interacts with BNLF1. Interacts with SQSTM1 upon TNF-alpha stimulation. May interacts with MAVS/IPS1. , |
Please login to post questions
Related products
