Price range: $268.00 through $328.00
Background：
Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression. [provided by RefSeq , Jul 2008] ,
| Size | 50μL/100μL |
| Target | SFRP4 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 40kD |
| MW(Observed) | 55kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:1000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1979R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | SFRP4 FRPHE |
| Protein Name | Secreted frizzled-related protein 4 (sFRP-4) (Frizzled protein, human endometrium) (FrpHE) |
| Other Name | |
| Species | Human |
| Gene ID | 6424 |
| Uniprot | Q6FHJ7, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q9Z1N6, |
| Species.2 | Rat |
| Gene ID.2 | |
| Uniprot.2 | Q9JLS4 |
| Cellular Localization | Secreted . Cytoplasmic in ovarian tumor cells. |
| Tissue Expression | Expressed in mesenchymal cells. Highly expressed in the stroma of proliferative endometrium. Expressed in cardiomyocytes. Shows moderate to strong expression in ovarian tumors with expression increasing as the tumor stage increases. In ovarian tumors, expression levels are inversely correlated with expression of CTNNB1 (at protein level). |
| Signaling_pathway | Environmental Information Processing >> Signal transduction >> Wnt signaling pathway |
| Research Areas | >>Wnt signaling pathway |
| Function | Disease:Associated with tumor-induced osteomalacia, a disorder in which there is an increase in renal phosphate excretion and a reduction in serum phosphate levels leading to hyperphosphaturia, hypophosphatemia and rickets.,Domain:The FZ domain is involved in binding with Wnt ligands.,Function:Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 may act as a regulator of adult uterine morphology and function. Increases apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling (By similarity). Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake.,induction:Increased levels in failing myocardium. Up-regulated in several tumor types including ostomalacia-associated tumors and endometrial and breast carcinomas.,similarity:Belongs to the secreted frizzled-related protein (sFRP) family.,similarity:Contains 1 FZ (frizzled) domain.,similarity:Contains 1 NTR domain.,tissue specificity:Expressed in mesenchymal cells. Highly expressed in the stroma of proliferative endometrium. Expressed in cardiomyocytes., |
