Specifications
| Size | 50μL/100μL |
| Target | GLYM |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 46kD |
| MW(Observed) | 43kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1858R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | SHMT2 |
| Protein Name | GLYM |
| Other Name | |
| Species | Human |
| Gene ID | 6472 |
| Uniprot | P34897 |
| Species.1 | |
| Gene ID.1 | |
| Uniprot.1 | |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Mitochondrion . Mitochondrion matrix, mitochondrion nucleoid . Mitochondrion inner membrane . Cytoplasm . Nucleus . Mainly localizes in the mitochondrion. Also found in the cytoplasm and nucleus as part of the BRISC complex (PubMed:24075985). . |
| Tissue Expression | |
| Signaling_pathway | |
| Research Areas | >>Glycine, serine and threonine metabolism; / >>Glyoxylate and dicarboxylate metabolism; / >>One carbon pool by folate; / >>Metabolic pathways; / >>Carbon metabolism; / >>Biosynthesis of amino acids; / >>Biosynthesis of cofactors; / >>Antifolate resistance |
| Function | Catalytic activity:5,10-methylenetetrahydrofolate + glycine + H(2)O = tetrahydrofolate + L-serine.,cofactor:Pyridoxal phosphate.,Function:Interconversion of serine and glycine.,miscellaneous:In eukaryotes there are two forms of the enzymes: a cytosolic one and a mitochondrial one.,pathway:One-carbon metabolism; tetrahydrofolate pathway.,similarity:Belongs to the SHMT family.,subunit:Homotetramer., |
