Name: U-Blot® TNF-R2 Rabbit mAb
SKU: WR5209
Price: 268 USD
Availability: InStock

Product details

Background:The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. The function of IAPs in TNF-receptor signalling is unknown, however, c-IAP1 is thought to potentiate TNF-induced apoptosis by the ubiquitination and degradation of TNF-receptor-associated factor 2, which mediates anti-apoptotic signals. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways. [provided by RefSeq, Jul 2008],

Specifications

Size	50μL/100μL
Target	TNF-R2
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, IP, ELISA
MW(Calculated)	48kD
MW(Observed)	60kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM1443R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	TNFRSF1B
Protein Name	Tumor necrosis factor receptor superfamily member 1B
Other Name	TNFRSF1B; / TNFBR; / TNFR2; / Tumor necrosis factor receptor superfamily member 1B; / Tumor necrosis factor receptor 2; / TNF-R2; / Tumor necrosis factor receptor type II; / TNF-RII; / TNFR-II; / p75; / p80 TNF-alpha receptor; / CD antigen CD120b; / Etanercept
Species	Human
Gene ID	7133
Uniprot	P20333,
Species.1	Mouse
Gene ID.1	21938
Uniprot.1	P25119,
Species.2	Rat
Gene ID.2	156767
Uniprot.2	Q80WY6
Cellular Localization	[Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Secreted.; [Tumor necrosis factor-binding protein 2]: Secreted.
Tissue Expression	Muscle,PNS,Urine,
Signaling_pathway	Organismal Systems >> Endocrine system >> Adipocytokine signaling pathway;Human Diseases >> Neurodegenerative disease >> Amyotrophic lateral sclerosis;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> TNF signaling pathway;Environmental Information Processing >> Signaling molecules and interaction >> Cytokine-cytokine receptor interaction;Environmental Information Processing >> Signaling molecules and interaction >> Viral protein interaction with cytokine and cytokine receptor
Research Areas	>>Cytokine-cytokine receptor interaction; / >>Viral protein interaction with cytokine and cytokine receptor; / >>TNF signaling pathway; / >>Adipocytokine signaling pathway; / >>Amyotrophic lateral sclerosis; / >>Pathways of neurodegeneration - multiple diseases; / >>Human immunodeficiency virus 1 infection
Function	Function:Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.,online information:Clinical information on Enbrel,pharmaceutical:Available under the name Enbrel (Immunex and Wyeth-Ayerst). Used to treat moderate to severe rheumatoid arthritis (RA). Enbrel consist of the extracellular ligand-binding portion of TNFRSF1B linked to an immunoglobulin Fc chain. It binds to TNF-alpha and blocks its interactions with receptors.,PTM:A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing.,PTM:Phosphorylated; mainly on serine residues and with a very low level on threonine residues.,similarity:Contains 4 TNFR-Cys repeats.,subunit:Binds to TRAF2.,