Name: U-Blot® Vimentin Mouse mAb (Ready to Use)
SKU: WM1747R
Price: 168 USD
Availability: InStock

U-Blot® Vimentin Mouse mAb (Ready to Use)

Ucallm_WM1747R

$168.00

Product details

Background：
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009],

Specifications

Target	Vimentin
Reactivity	Human, Mouse, Rat
Applications	IHC
MW(Calculated)
MW(Observed)
Host Species	Mouse
Isotype	IgG2b,Kappa
Conjugate/Modification	Unmodified
Modification
Recommended Dilution Ratio	Ready to use for IHC / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004)
Formulation	The prediluted ready-to-use antibody is diluted in phosphate buffer saline containing stabilizing protein and 0.05% Proclin 300
Source
Purification	The antibody was affinity-purified from ascites by affinity-chromatography using specific immunogen.
Purity
Storage	2°C to 8°C/1 year,Ship by ice bag
Concentration
Clonality	Monoclonal
Clone Number	ABT019
Immunogen	Synthesized peptide derived from human Vimentin AA range: 400-466
Sequence
Specificity	The antibody can specifically recognize human Vimentin protein.
Gene Name	VIM
Protein Name	Vimentin
Other Name	Vimentin
Species	Human
Gene ID-1	7431
Uniprot	P08670,
Species.1	Mouse
Gene ID-2	22352
Uniprot.1	P20152,
Species.2	Rat
Gene ID-3	81818
Uniprot.2	P31000
Organism-4
Gene ID-4
SwissProt-4
Background	This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009],
Cellular Localization	Cytoplasmic
Tissue Expression	Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
Signaling_pathway	Human Diseases >> Cancer: overview >> MicroRNAs in cancer
Research Areas	>>Epstein-Barr virus infection; / >>MicroRNAs in cancer
Function	Function:Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.,online information:Vimentin entry,PTM:One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized.,sequence Caution:Intron retention.,similarity:Belongs to the intermediate filament family.,subunit:Homopolymer. Interacts with HCV core protein. Interacts with LGSN and SYNM.,tissue specificity:Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.,
RRID