SKU: WR5958 Categories: ,

U-Blot® XDH Rabbit mAb

Price range: $268.00 through $328.00

SKU: WR5958-50
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Product details

Background:
Xanthine dehydrogenase belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Xanthine dehydrogenase can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. Defects in xanthine dehydrogenase cause xanthinuria , may contribute to adult respiratory stress syndrome , and may potentiate influenza infection through an oxygen metabolite-dependent mechanism. [provided by RefSeq , Jan 2014] ,

Specifications

Size50μL/100μL
TargetXDH
ReactivityHuman, Mouse, Rat
ApplicationsWB, IHC, IF, ELISA
MW(Calculated)146kD
MW(Observed)146kD
Host SpeciesRabbit
IsotypeIgG,Kappa
Conjugate/ModificationUnmodified
Recommended Dilution RatioIHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationPBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
PurificationRecombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
ClonalityMonoclonal
Clone NumberRM1896R
ImmunogenThe specific immunogen used to produce this antibody is proprietary information.
SpecificityEndogenous
Gene NameXDH XDHA
Protein NameXanthine dehydrogenase/oxidase [Includes: Xanthine dehydrogenase (XD) (Xanthine oxidoreductase) (XOR)]
Other Name
SpeciesHuman
Gene ID7498
UniprotP47989,
Species.1Mouse
Gene ID.1
Uniprot.1Q00519,
Species.2Rat
Gene ID.2
Uniprot.2P22985
Cellular LocalizationCytoplasm . Peroxisome . Secreted.
Tissue ExpressionDetected in milk (at protein level).
Signaling_pathwayCellular Processes >> Transport and catabolism >> Peroxisome
Research Areas>>Purine metabolism; / >>Caffeine metabolism; / >>Drug metabolism - other enzymes; / >>Metabolic pathways; / >>Nucleotide metabolism; / >>Peroxisome
FunctionCatalytic activity:Hypoxanthine + NAD(+) + H(2)O = xanthine + NADH.,Catalytic activity:Xanthine + H(2)O + O(2) = urate + H(2)O(2).,Catalytic activity:Xanthine + NAD(+) + H(2)O = urate + NADH.,cofactor:Binds 2 2Fe-2S clusters.,cofactor:FAD.,cofactor:Molybdopterin.,Disease:Defects in XDH are the cause of xanthinuria type 1 (XU1) [MIM:278300]. Xanthinuria is characterized by excretion of very large amounts of xanthine in the urine and a tendency to form xanthine stones. Uric acid is strikingly diminished in serum and urine. XU1 is due to isolated xanthine dehydrogenase. XU1 patients can metabolize allopurinol.,Disease:May contribute to adult respiratory stress syndrome (ARDS) and may potentiate influenza infection through an oxygen metabolite-dependent mechanism.,Function:This enzyme can be converted from the dehydrogenase form (D) to the oxidase form (O) irreversibly by proteolysis or reversibly through the oxidation of sulfhydryl groups.,similarity:Belongs to the xanthine dehydrogenase family.,similarity:Contains 1 2Fe-2S ferredoxin-type domain.,similarity:Contains 1 FAD-binding PCMH-type domain.,subunit:Homodimer. Interacts with BTN1A1.,
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