Name: Ub Polyclonal Antibody
SKU: WP15552
Price: 268 USD
Availability: InStock

Product details

Background:

Ubiquitin is a highly conserved nuclear and cytoplasmic protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein L40 at the C terminus, a C-terminal extension protein (CEP). Multiple processed pseudogenes derived from this gene are present in the genome. [provided by RefSeq, Jul 2008],

Specifications

Target	Ubiquitin
Reactivity	Human, Mouse, Rat
Applications	IF, WB, IHC, ELISA
MW(Calculated)
MW(Observed)	80kD,50kD
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	Unmodified
Modification
Recommended Dilution Ratio	IF 1:50-200; WB 1:500-1:2000; IHC: 1:100-1:300; ELISA 1:10000; Not yet tested in other applications; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	Synthesized peptide derived from the N-terminal region of human Ub.
Sequence
Specificity	Ub Polyclonal Antibody detects endogenous levels of Ub protein.
Gene Name	UBA52/RPS27A/UBB/UBC
Protein Name	Ubiquitin
Other Name	UBB; / Polyubiquitin-B; / UBC; / Polyubiquitin-C; / RPS27A; / UBA80; / UBCEP1; / Ubiquitin-40S ribosomal protein S27a; / Ubiquitin carboxyl extension protein 80; / UBA52; / UBCEP2; / Ubiquitin-60S ribosomal protein L40; / CEP52; / Ubiquitin A-52 residue ribosomal protein fusion product 1
Species	Human
Gene ID-1	7311
Uniprot	P0CG47;P0CG48;P62979;P62987
Species.1
Gene ID-2
Uniprot.1
Species.2
Gene ID-3
Uniprot.2
Organism-4
Gene ID-4
SwissProt-4
Background	Ubiquitin is a highly conserved nuclear and cytoplasmic protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein L40 at the C terminus, a C-terminal extension protein (CEP). Multiple processed pseudogenes derived from this gene are present in the genome. [provided by RefSeq, Jul 2008],
Cellular Localization	[Ubiquitin]: Cytoplasm . Nucleus .; [60S ribosomal protein L40]: Cytoplasm .
Tissue Expression	Brain,Epithelium,Fetal brain cortex,Liver,L
Signaling_pathway	Cellular Processes >> Transport and catabolism >> Autophagy - animal;Cellular Processes >> Transport and catabolism >> Mitophagy - animal;Human Diseases >> Neurodegenerative disease >> Parkinson disease;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Genetic Information Processing >> Folding, sorting and degradation >> Ubiquitin mediated proteolysis
Research Areas	>>Ribosome; / >>Ubiquitin mediated proteolysis; / >>Mitophagy - animal; / >>Parkinson disease; / >>Pathways of neurodegeneration - multiple diseases; / >>Shigellosis; / >>Kaposi sarcoma-associated herpesvirus infection; / >>Coronavirus disease - COVID-19
Function	Function:Protein modifier which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Attachment to proteins as a Lys-48-linked polymer usually leads to their degradation by proteasome. Attachment to proteins as a monomer or as an alternatively linked polymer does not lead to proteasomal degradation and may be required for numerous functions, including maintenance of chromatin structure, regulation of gene expression, stress response, ribosome biogenesis and DNA repair.,miscellaneous:This ribosomal protein is synthesized as a C-terminal extension protein (CEP) of ubiquitin.,miscellaneous:Ubiquitin is synthesized as a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains. In some species there is a final amino-acid after the last repeat, here in human a Val. Some ubiquitin genes contain a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27a).,PTM:Several types of polymeric chains can be formed, depending on the lysine used for the assembly.,similarity:Belongs to the ribosomal protein L40e family.,similarity:Belongs to the ribosomal protein S27Ae family.,similarity:Belongs to the ubiquitin family.,
RRID

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Ub Polyclonal Antibody

Product details

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Ub Polyclonal Antibody

Product details

Specifications

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