Ubr1 Polyclonal Antibody
Ucallm_WP14927-50
Price range: $268.00 through $328.00
Product details
Background:
The N-end rule pathway is one proteolytic pathway of the ubiquitin system. The recognition component of this pathway, encoded by this gene, binds to a destabilizing N-terminal residue of a substrate protein and participates in the formation of a substrate-linked multiubiquitin chain. This leads to the eventual degradation of the substrate protein. The protein described in this record has a RING-type zinc finger and a UBR-type zinc finger. Mutations in this gene have been associated with Johanson-Blizzard syndrome. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | Ubr1 |
| Reactivity | Human, Mouse |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 200kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human UBR1. AA range:821-870 |
| Specificity | Ubr1 Polyclonal Antibody detects endogenous levels of Ubr1 protein. |
| Gene Name | UBR1 |
| Protein Name | E3 ubiquitin-protein ligase UBR1 |
| Other Name | UBR1; / E3 ubiquitin-protein ligase UBR1; / N-recognin-1; / Ubiquitin-protein ligase E3-alpha-1; / Ubiquitin-protein ligase E3-alpha-I |
| Species | Human |
| Gene ID | 197131 |
| Uniprot | Q8IWV7, |
| Species.1 | Mouse |
| Gene ID.1 | 22222 |
| Uniprot.1 | O70481 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm, cytosol . |
| Tissue Expression | Broadly expressed, with highest levels in skeletal muscle, kidney and pancreas. Present in acinar cells of the pancreas (at protein level). |
| Signaling_pathway | |
| Research Areas | |
| Function | developmental stage:Expressed in fetal pancreas.,Disease:Defects in UBR1 are a cause of Johanson-Blizzard syndrome (JBS) [MIM:243800]. This disorder includes congenital exocrine pancreatic insufficiency, multiple malformations such as nasal wing aplasia, and frequent mental retardation. Pancreas of individuals with JBS do not express UBR1 and show intrauterine-onset destructive pancreatitis.,Domain:The RING-H2 zinc finger is an atypical RING finger with a His ligand in place of the fourth Cys of the classical motif.,Function:E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. May be involved in pancreatic homeostasis.,pathway:Protein modification; protein ubiquitination.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the UBR1 family.,similarity:Contains 1 RING-type zinc finger.,similarity:Contains 1 UBR-type zinc finger.,subunit:Interacts with RECQL4.,tissue specificity:Broadly expressed, with highest levels in skeletal muscle, kidney and pancreas. Present in acinar cells of the pancreas (at protein level)., |
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