Product details
This is a rapid and reversible staining for locating protein bands on Western blot and for comparing protein band intensity. It produces pink reddish stained bands on Western blot membranes.The stain is very useful because it does not have a deleterious effect on the property of proteins.It is also a common method for locating polypeptides on Western blots for protein sequencing. The stain binds to proteins on the membrane reversibly and works well for nitrocellulose and PVDF membranes.It is not recommended for positively charged membranes such as nylon membrane.
Protocol
- Incubate the membrane with the stain solution for 1 min to up to 30 min with gentle agitation.
- Rinse the membrane in deionized water until the background is clean (usually it takes less than5 min). The stain can be completely removed from the protein bands by continuing washing with deionized water.