Product details
Proteins account for about 80% of keratinized animal tissues that include but not limited to hair, nail, horn and wool. These tissues contain mainly hard α-keratins made of several distinctive types of protein with molecular weight ranging from 10-135Kda. Majority of the proteins are ranging from 10-65Kda. Several methods have been reported for protein extraction from keratinized animal tissues. The extraction buffer usually contains high concentration of urea, thiourea, guanidine hydrochloride or a combination of these strong denaturants. Protein extraction with high concentration denaturants is relatively effective but the concentration of extracted protein is usually low due to larger extraction buffer/tissue ratio. Extracted proteins need to be concentrated prior to further analysis. The presence of high concentration of denaturants may also interfere with downstream application. W2034 is designed to effectively extract proteins from keratinized animal tissues without using strong denaturants mentioned above. The extracted protein can be directly loaded onto SDS-PAGE without concentration. The extraction buffer of this kit contains 0.5% SDS and other chemicals. Extracted protein concentration is 1-2 mg/ml.
Application
This kit is designed to extract proteins from keratinized animal tissues for applications such as SDS- PAGE, immunoblottings, ELISA and other applications.
