SKU: WP3369 Categories: ,

VPS11 Polyclonal Antibody

Price range: $268.00 through $328.00

SKU: WP3369-100
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Product details

Background:

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps11 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014],

Specifications

Size50μL/100μL
TargetVPS11
ReactivityHuman, Mouse
ApplicationsWB, ELISA
MW(Calculated)
MW(Observed)103kD
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:500-2000; ELISA 1:5000-20000
FormulationLiquid in PBS containing 50% glycerol,0.5% BSA and 0.02% sodium azide.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenSynthesized peptide derived from part region of human protein
SpecificityVPS11 Polyclonal Antibody detects endogenous levels of protein.
Gene NameVPS11 RNF108 PP3476
Protein NameVacuolar protein sorting-associated protein 11 homolog (hVPS11) (RING finger protein 108)
Other Name
SpeciesHuman
Gene ID55823
UniprotQ9H270,
Species.1Mouse
Gene ID.1
Uniprot.1Q91W86
Species.2
Gene ID.2
Uniprot.2
Cellular LocalizationEndosome . Late endosome membrane; Peripheral membrane protein; Cytoplasmic side . Lysosome membrane; Peripheral membrane protein; Cytoplasmic side . Early endosome . Cytoplasmic vesicle . Cytoplasmic vesicle, autophagosome . Cytoplasmic vesicle, clathrin-coated vesicle .
Tissue ExpressionUbiquitous. Expression was highest in heart and low in lung.
Signaling_pathwayCellular Processes >> Transport and catabolism >> Autophagy - animal
Research Areas>>Salmonella infection
FunctionFunction:May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.,similarity:Belongs to the VPS11 family.,similarity:Contains 1 clathrin repeat.,similarity:Contains 1 RING-type zinc finger.,subcellular location:Cytoplasmic, peripheral membrane protein associated with late endosomes/lysosomes.,subunit:Large hetero-oligomeric complex together with VPS16, VPS18 and VPS33A. Interacts with STX7.,tissue specificity:Ubiquitous. Expression was highest in heart and low in lung.,
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