U-Blot® Zap-70 (Phospho Tyr319)/Syk (Phospho Tyr352) Rabbit mAb
WR4058 Immunohistochemical analysis of paraffin-embedded Human kidney tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
WR4058 Western blot analysis of lysates from Hela cells, using SDHA Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.
Price range: $288.00 through $368.00
Product details
Background:ZAP70 gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],SYK gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010],
Specifications
| Size | 50μL/100μL |
| Target | ZAP-70/Syk Phospho Tyr319/Tyr352 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IF, ELISA |
| MW(Calculated) | 70kD |
| MW(Observed) | 70kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | phosphate |
| Recommended Dilution Ratio | IHC 1:200-1000; WB 1:1000-5000; IF 1:200-1000; ELISA 1:5000-20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0710R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | The antibody detects endogenous ZAP-70/Syk(Phospho AE13) protein. |
| Gene Name | ZAP70/SYK |
| Protein Name | Tyrosine-protein kinase ZAP-70/Tyrosine-protein kinase SYK |
| Other Name | ZAP70; / SRK; / Tyrosine-protein kinase ZAP-70; / 70 kDa zeta-chain associated protein; / Syk-related tyrosine kinase; / SYK; / Tyrosine-protein kinase SYK; / Spleen tyrosine kinase; / p72-Syk |
| Species | Human |
| Gene ID | 6389 |
| Uniprot | P43403;P43405, |
| Species.1 | Mouse |
| Gene ID.1 | 22637;20963 |
| Uniprot.1 | P43404;P48025, |
| Species.2 | Rat |
| Gene ID.2 | 157074 |
| Uniprot.2 | Q920L2 |
| Cellular Localization | Cytoplasm . Cell membrane; Peripheral membrane protein . In quiescent T-lymphocytes, it is cytoplasmic. Upon TCR activation, it is recruited at the plasma membrane by interacting with CD247/CD3Z. Colocalizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes (By similarity). cytosol . |
| Tissue Expression | Adipocyte,Brain,Colon,Heart,Liver,Placenta, |
| Signaling_pathway | Human Diseases >> Neurodegenerative disease >> Alzheimer disease;Human Diseases >> Neurodegenerative disease >> Parkinson disease;Human Diseases >> Neurodegenerative disease >> Amyotrophic lateral sclerosis;Human Diseases >> Neurodegenerative disease >> Huntington disease;Human Diseases >> Neurodegenerative disease >> Prion disease;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases |
| Research Areas | >>Citrate cycle (TCA cycle); / >>Oxidative phosphorylation; / >>Metabolic pathways; / >>Carbon metabolism; / >>Thermogenesis; / >>Non-alcoholic fatty liver disease; / >>Alzheimer disease; / >>Parkinson disease; / >>Amyotrophic lateral sclerosis; / >>Huntington disease; / >>Prion disease; / >>Pathways of neurodegeneration - multiple diseases; / >>Chemical carcinogenesis - reactive oxygen species; / >>Diabetic cardiomyopathy |
| Function | Catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,Disease:Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.,Domain:The SH2 domain binds to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z.,Function:Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.,online information:ZAP70 mutation db,PTM:Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 SH2 domains.,subcellular location:After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic.,subunit:Interacts with SLA2 when it is phosphorylated. Interacts with CD3Z and with phosphorylated NFAM1. Interacts with CBLB (By similarity). Interacts with CBL and SLA when it is phosphorylated. The association with SLA (or SLA2) and CBL probably leads to its destruction. Interacts with SHB. Interacts with DEF6 (By similarity). Interacts with FCRL3.,tissue specificity:Expressed in T- and natural killer cells., |
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