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- Experimental steps required
  Sample preparation
  Preparation of polyacrylamide gel
  Loading and running the gel
  Setup of transfer apparatus
  Transferring from the gel to the membrane
  Verification and blocking
  Antibody incubation
  Membrane stripping (optional)
  Detection and data analysis
  
  Instruments
  Thermal control
  Electrophoresis cell
  Centrifuges
  Mixing
  Spectrometer
  Electrophoresis power supply series
  Imaging luminescence series
  Western blot transfer system
  
  Reagents
  Protein separation and extraction kit
  Lysis buffer
  Running buffer
  Transfer buffer
  Blocking solution
  Detection substrate
  SDS-PAGE gel
  Antibody
  
  Consumables
  Blotting membrane
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Tag: Antibody analysis

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Laboratory Technology

Western Blot Protocol: Step-by-Step Guide from Sample Prep to Detection

Standard Flowchart of Western Blot

Follow our comprehensive Western Blot Protocol guide for precise sample preparation and detection techniques

June 12, 2025

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Products
- Experimental steps required
  Sample preparation
  Preparation of polyacrylamide gel
  Loading and running the gel
  Setup of transfer apparatus
  Transferring from the gel to the membrane
  Verification and blocking
  Antibody incubation
  Membrane stripping (optional)
  Detection and data analysis
  
  Instruments
  Thermal control
  Electrophoresis cell
  Centrifuges
  Mixing
  Spectrometer
  Electrophoresis power supply series
  Imaging luminescence series
  Western blot transfer system
  
  Reagents
  Protein separation and extraction kit
  Lysis buffer
  Running buffer
  Transfer buffer
  Blocking solution
  Detection substrate
  SDS-PAGE gel
  Antibody
  
  Consumables
  Blotting membrane
Services
Applications
Resources
Partners

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