Specifications
| Size | 50μL/100μL |
| Target | LAT1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 55kD |
| MW(Observed) | 40kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1462R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | SLC7A5 CD98LC LAT1 MPE16 |
| Protein Name | Large neutral amino acids transporter small subunit 1 (4F2 light chain) (4F2 LC) (4F2LC) (CD98 light chain) (Integral membrane protein E16) (L-type amino acid transporter 1) (hLAT1) (Solute carrier family 7 member 5) (y+ system cationic amino acid transporter) |
| Other Name | |
| Species | Human |
| Gene ID | 8140 |
| Uniprot | Q01650, |
| Species.1 | Mouse |
| Gene ID.1 | 20539 |
| Uniprot.1 | Q9Z127, |
| Species.2 | Rat |
| Gene ID.2 | 20539 |
| Uniprot.2 | Q63016 |
| Cellular Localization | Apical cell membrane; Multi-pass membrane protein . Cell membrane; Multi-pass membrane protein . Lysosome membrane; Multi-pass membrane protein . Located to the plasma membrane by SLC3A2/4F2hc (PubMed:9751058). Localized to the apical membrane of placental syncytiotrophoblastic cells (PubMed:11742812). Recruited to lysosomes by LAPTM4B (PubMed:25998567). Expressed in both luminal and abluminal membranes of brain capillary endothelial cells (By similarity). . |
| Tissue Expression | Detected in placenta, in the syncytiotrophoblast layer (at protein level) (PubMed:11389679). Expressed abundantly in adult lung, liver, brain, skeletal muscle, placenta, bone marrow, testis, resting lymphocytes and monocytes, and in fetal liver. Weaker expression in thymus, cornea, retina, peripheral leukocytes, spleen, kidney, colon and lymph node. During gestation, expression in the placenta was significantly stronger at full-term than at the mid-trimester stage. Also expressed in all human tumor cell lines tested and in the astrocytic process of primary astrocytic gliomas. Expressed in retinal endothelial cells and in the intestinal epithelial cell line Caco-2. |
| Signaling_pathway | Human Diseases >> Cancer: overview >> Central carbon metabolism in cancer;Environmental Information Processing >> Signal transduction >> mTOR signaling pathway |
| Research Areas | >>mTOR signaling pathway; / >>Central carbon metabolism in cancer |
| Function |
