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Background:Enables polyubiquitin modification-dependent protein binding activity. Involved in defense response to bacterium; protein linear polyubiquitination; and regulation of signal transduction. Located in cytosol. Part of LUBAC complex. [provided by Alliance of Genome Resources, Jul 2025]
| Target | SHARPIN |
| Reactivity | Human |
| Applications | WB,IF,IP,ELISA |
| MW(Calculated) | 40kD |
| MW(Observed) | 43kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:1000-1:5000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200; |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Source | |
| Purification | Protein A |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Clonality | Monoclonal |
| Clone Number | PT1596R |
| Specificity | Endogenous |
| Gene Name | SHARPIN SIPL1 PSEC0216 |
| Protein Name | Sharpin |
| Other Name | Shank-associated RH domain-interacting protein;Shank-interacting protein-like 1;hSIPL1 |
| Organism-1 | Human |
| Gene ID-1 | 81858 |
| SwissProt-1 | Q9H0F6 |
| Organism-2 | Mouse |
| Gene ID-2 | 106025 |
| SwissProt-2 | Q91WA6 |
| Organism-3 | Rat |
| Gene ID-3 | 81859 |
| SwissProt-3 | Q9EQL9 |
| Function | Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation . LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways . Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation . LUBAC is recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex . The LUBAC complex is also involved in innate immunity by conjugating linear polyubiquitin chains at the surface of bacteria invading the cytosol to form the ubiquitin coat surrounding bacteria . LUBAC is not able to initiate formation of the bacterial ubiquitin coat, and can only promote formation of linear polyubiquitins on pre-existing ubiquitin . The bacterial ubiquitin coat acts as an 'eat-me' signal for xenophagy and promotes NF-kappa-B activation . Together with OTULIN, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis . |
| Cellular Localization | Cytoplasm, cytosol . Cell junction, synapse . Enriched at synaptic sites in mature neurons where it colocalizes with SHANK1. . |
| Tissue Expression | Highly expressed in skeletal muscle and placenta and at lower levels in brain, heart, colon without mucosa, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes. Up-regulated in various tumor tissues such as kidney, liver, ovary and pancreas tumors. |
| Unnamed: 31 | |
| SwissProt-3.1 | |
| Organism-4 | |
| Gene ID-4 | |
| SwissProt-4 | |
| Cellular Localization.1 |
