Name: U-Blot® Cleaved PARP1 Rabbit mAb
SKU: WR4004
Price: 268 USD
Availability: InStock

U-Blot® Cleaved PARP1 Rabbit mAb

WR4004

RAW264.7 cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with primary antibody 1:1000. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

WR4004 RAW264.7 cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with primary antibody 1:1000. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

Price range: $268.00 through $328.00

Size

100μl

50μl

Clear

SKU: WR4004-50

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Product details

Background:This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008],

Specifications

Size	50μL/100μL
Target	PARP
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, ELISA
MW(Calculated)	113kD
MW(Observed)	25kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1000; WB 1:500-5000; IF 1:200-1000; ELISA 1:5000-20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0046R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	This antibody detects endogenous levels of Cleaved PARP1.
Gene Name	PARP1
Protein Name	Poly [ADP-ribose] polymerase 1
Other Name	PARP1; / ADPRT; / PPOL; / Poly [ADP-ribose] polymerase 1; / PARP-1; / ADP-ribosyltransferase diphtheria toxin-like 1; / ARTD1; / NAD; / +; / ADP-ribosyltransferase 1; / ADPRT 1; / Poly[ADP-ribose] synthase 1
Species	Human
Gene ID	142
Uniprot	P09874,
Species.1	Mouse
Gene ID.1
Uniprot.1	P11103
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Cytoplasmic, Nuclear
Tissue Expression	Brain,Colon carcinoma,Fibroblast,Lung,Ovarian carcinoma,Skin,
Signaling_pathway	Cellular Processes >> Cell growth and death >> Apoptosis;Cellular Processes >> Cell growth and death >> Necroptosis;Environmental Information Processing >> Signal transduction >> NF-kappa B signaling pathway
Research Areas	>>Base excision repair; / >>NF-kappa B signaling pathway; / >>Apoptosis; / >>Necroptosis; / >>Diabetic cardiomyopathy
Function	Catalytic activity:NAD(+) + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.,Function:Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.,miscellaneous:The ADP-D-ribosyl group of NAD(+) is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units.,PTM:Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.,PTM:Poly-ADP-ribosylated by PARP2.,similarity:Contains 1 BRCT domain.,similarity:Contains 1 PARP alpha-helical domain.,similarity:Contains 1 PARP catalytic domain.,similarity:Contains 2 PARP-type zinc fingers.,subunit:Component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LIG3. Homo- and heterodimer with PARP2. Interacts with PARP3, APTX and SRY. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70. Interacts with TIAM2 and ZNF423.,