Name: U-Blot® NQO1 Rabbit mAb
SKU: WR4020
Price: 268 USD
Availability: InStock

U-Blot® NQO1 Rabbit mAb

WR4020

Immunohistochemical analysis of paraffin-embedded Human kidney tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).

WR4020 Immunohistochemical analysis of paraffin-embedded Human kidney tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).

WR4020 Immunohistochemical analysis of paraffin-embedded Rat kidney tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).

WR4020 Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).

Western blot analysis of lysates from Hela cells, using NQO1 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.

WR4020Western blot analysis of lysates from Hela cells, using NQO1 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.

Price range: $268.00 through $328.00

Size

100μl

50μl

Clear

SKU: WR4020-50

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Product details

Background:This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008],

Specifications

Size	50μL/100μL
Target	NQO1
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, IP, ELISA
MW(Calculated)	31kD
MW(Observed)	31kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1:1000; WB 1:1000-1:5000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200, / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0071R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	NQO1
Protein Name	NQO1
Other Name	NQO1; / DIA4; / NMOR1; / NAD; / P; / H dehydrogenase [quinone] 1; / Azoreductase; / DT-diaphorase; / DTD; / Menadione reductase; / NAD; / P; / H:quinone oxidoreductase 1; / Phylloquinone reductase; / Quinone reductase 1; / QR1
Species	Human
Gene ID	1728
Uniprot	P15559,
Species.1	Mouse
Gene ID.1
Uniprot.1	Q64669
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Cytoplasm
Tissue Expression	Colon,Liver,Pooled,
Signaling_pathway	Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: specific types >> Hepatocellular carcinoma
Research Areas
Function	Catalytic activity:NAD(P)H + a quinone = NAD(P)(+) + a hydroquinone.,cofactor:FAD.,enzyme regulation:Inhibited by dicoumarol.,Function:The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.,induction:By dioxin.,mass spectrometry: PubMed:11735396,miscellaneous:Quinone reductase accepts electrons from both NADH and NADPH with equal efficiency.,polymorphism:The Ser-187 polymorphism may be linked to susceptibility to forms of cancers.,similarity:Belongs to the NAD(P)H dehydrogenase (quinone) family.,subunit:Homodimer.,