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Home/Reagents/Antibody/Primary antibody/Monoclonal Antibody/Rabbit mAb/U-Blot® RSK2 Rabbit mAb
SKU: WR4472 Categories: ,

U-Blot® RSK2 Rabbit mAb

Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:2500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody.
WR4472 Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:2500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody.
A549 cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with primary antibody 1:1000. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
WR4472 A549 cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with primary antibody 1:1000. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

Price range: $268.00 through $328.00

SKU: WR4472-50
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Product details

Background:ribosomal protein S6 kinase A3(RPS6KA3) Homo sapiens This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS). [provided by RefSeq, Jul 2008],

Specifications

Size50μL/100μL
TargetRSK2
ReactivityHuman, Mouse, Rat
ApplicationsWB, IHC, IF, IP, ELISA
MW(Calculated)84kD
MW(Observed)84kD
Host SpeciesRabbit
IsotypeIgG,Kappa
Conjugate/ModificationUnmodified
Recommended Dilution RatioIHC 1:1000-1:5000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationPBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
PurificationRecombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
ClonalityMonoclonal
Clone NumberRM0827R
ImmunogenThe specific immunogen used to produce this antibody is proprietary information.
SpecificityEndogenous
Gene NameRPS6KA3 ISPK1 MAPKAPK1B RSK2
Protein NameRSK2
Other NameRibosomal protein S6 kinase alpha-3; / S6K-alpha-3; / 90 kDa ribosomal protein S6 kinase 3; / p90-RSK 3; / p90RSK3; / Insulin-stimulated protein kinase 1; / ISPK-1; / MAP kinase-activated protein kinase 1b; / MAPK-activated protein kinase 1b; / MAPKAP kinase 1b; / MAPKAPK-1b; / Ribosomal S6 kinase 2; / RSK-2; / pp90RSK2
SpeciesHuman
Gene ID6777
UniprotP51812,
Species.1Mouse
Gene ID.1110651
Uniprot.1P18654,
Species.2Rat
Gene ID.225126
Uniprot.2P52632
Cellular LocalizationNucleus . Cytoplasm .
Tissue ExpressionBrain,Epithelium,Lymph,Placenta,
Signaling_pathwayCellular Processes >> Cell growth and death >> Necroptosis;Organismal Systems >> Immune system >> Th1 and Th2 cell differentiation;Organismal Systems >> Immune system >> Th17 cell differentiation;Organismal Systems >> Immune system >> Chemokine signaling pathway;Organismal Systems >> Endocrine system >> Prolactin signaling pathway;Organismal Systems >> Endocrine system >> Growth hormone synthesis, secretion and action;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: specific types >> Acute myeloid leukemia;Human Diseases >> Cancer: specific types >> Chronic myeloid leukemia;Human Diseases >> Cancer: specific types >> Non-small cell lung cancer;Environmental Information Processing >> Signal transduction >> ErbB signaling pathway;Environmental Information Processing >> Signal transduction >> JAK-STAT signaling pathway
Research Areas>>ErbB signaling pathway; / >>Chemokine signaling pathway; / >>Necroptosis; / >>JAK-STAT signaling pathway; / >>Th1 and Th2 cell differentiation; / >>Th17 cell differentiation; / >>Prolactin signaling pathway; / >>AGE-RAGE signaling pathway in diabetic complications; / >>Growth hormone synthesis, secretion and action; / >>Hepatitis B; / >>Measles; / >>Human T-cell leukemia virus 1 infection; / >>Pathways in cancer; / >>Viral carcinogenesis; / >>Chemical carcinogenesis - receptor activation; / >>Chronic myeloid leukemia; / >>Acute myeloid leukemia; / >>Non-small cell lung cancer
FunctionCatalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,Disease:Defects in RPS6KA3 are the cause of Coffin-Lowry syndrome (CLS) [MIM:303600]; an X-linked dominant disorder characterized by severe mental retardation with facial and digital dysmorphisms, and progressive skeletal deformations.,enzyme regulation:Activated by multiple phosphorylations on threonine and serine residues.,Function:Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.,PTM:Autophosphorylated on Ser-386, as part of the activation process.,PTM:Ser-227 phosphorylation promotes Ser-386 phosphorylation and leads to basal activation. Full activation by growth factors requires additional phosphorylation on Ser-369.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 2 protein kinase domains.,subunit:Forms a complex with either ERK1 or ERK2 in quiescent cells. Transiently dissociates following mitogenic stimulation (By similarity). Interacts with NFATC4.,tissue specificity:Expressed in many tissues, highest levels in skeletal muscle.,
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