Name: U-Blot® Drosha Rabbit mAb
SKU: WR4660
Price: 268 USD
Availability: InStock

U-Blot® Drosha Rabbit mAb

Western Blot analysis using Hela lysates,primary antibody was diluted at 1:1000, 4°over night, Dylight 800 secondary antibody(WS0446)was diluted at 1:10000, 37° 1hour.

WR4660 Western Blot analysis using Hela lysates,primary antibody was diluted at 1:1000, 4°over night, Dylight 800 secondary antibody(WS0446)was diluted at 1:10000, 37° 1hour.

Price range: $268.00 through $328.00

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100μl

50μl

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SKU: WR4660-50

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Product details

Background:drosha ribonuclease III(DROSHA) Homo sapiens This gene encodes a ribonuclease (RNase) III double-stranded RNA-specific ribonuclease and subunit of the microprocessor protein complex, which catalyzes the initial processing step of microRNA (miRNA) synthesis. The encoded protein cleaves the stem loop structure from the primary microRNA (pri-miRNA) in the nucleus, yielding the precursor miRNA (pre-miRNA), which is then exported to the cytoplasm for further processing. In a human cell line lacking a functional copy of this gene, canonical miRNA synthesis is reduced. Somatic mutations in this gene have been observed in human patients with kidney cancer. [provided by RefSeq, Sep 2016],

Specifications

Size	50μL/100μL
Target	Drosha
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, IP, ELISA
MW(Calculated)	159kD
MW(Observed)	159kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0944R
Immunogen	Synthesized peptide derived from human N-ternal Drosha.
Specificity	Endogenous
Gene Name	DROSHA
Protein Name	Ribonuclease 3
Other Name	DROSHA; / RN3; / RNASE3L; / RNASEN; / Ribonuclease 3; / Protein Drosha; / Ribonuclease III; / RNase III; / p241
Species	Human
Gene ID	29102
Uniprot	Q9NRR4,
Species.1	Mouse
Gene ID.1	14000
Uniprot.1	Q5HZJ0
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Nucleus . Nucleus, nucleolus . A fraction is translocated to the nucleolus during the S phase of the cell cycle. Localized in GW bodies (GWBs), also known as P-bodies. .
Tissue Expression	Ubiquitous.
Signaling_pathway
Research Areas	>>Ribosome biogenesis in eukaryotes; / >>Proteoglycans in cancer
Function	Catalytic activity:Endonucleolytic cleavage to 5'-phosphomonoester.,cofactor:Magnesium or manganese.,Function:Executes the initial step of microRNA (miRNA) processing in the nucleus, that is cleavage of pri-miRNA to release pre-miRNA. Involved in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA.,online information:The dark side of RNA -Issue 87 of October 2007,similarity:Contains 1 DRBM (double-stranded RNA-binding) domain.,similarity:Contains 2 RNase III domains.,subcellular location:A fraction is translocated to the nucleolus during the S phase of the cell cycle.,subunit:Interacts with Sp1.,tissue specificity:Ubiquitous.,

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U-Blot® Drosha Rabbit mAb

Product details

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U-Blot® Drosha Rabbit mAb

Product details

Specifications

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