SKU: WM1096 Categories: ,

HSC70 Mouse mAb [7G12]

Price range: $268.00 through $328.00

SKU: WM1096-50
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Product details

Background:
This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011],

Specifications

Size50μL/100μL
TargetHSC70
ReactivityHuman, Mouse, Rat
ApplicationsIHC, WB
MW(Calculated)
MW(Observed)73kD
Host SpeciesMouse
Isotype
Conjugate/ModificationUnmodified
Recommended Dilution RatioIHC1:50-200; WB 1:1000-2000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationThe antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityMonoclonal
Clone Number7G12
ImmunogenSynthesized peptide derived from human HSC70
SpecificityThis antibody detects endogenous levels of HSC70 at Human, Mouse,Rat
Gene NameHSPA8 HSC70 HSP73 HSPA10
Protein NameHeat shock cognate 71 kDa protein
Other NameHSPA8; / HSC70; / HSP73; / HSPA10; / Heat shock cognate 71 kDa protein; / Heat shock 70 kDa protein 8
SpeciesHuman
Gene ID3312
UniprotP11142,
Species.1Mouse
Gene ID.115481
Uniprot.1P63017,
Species.2Rat
Gene ID.224468
Uniprot.2P63018
Cellular LocalizationCytoplasm. Melanosome. Nucleus, nucleolus. Cell membrane. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock.
Tissue ExpressionUbiquitous.
Signaling_pathwayOrganismal Systems >> Immune system >> Antigen processing and presentation;Organismal Systems >> Endocrine system >> Estrogen signaling pathway;Organismal Systems >> Aging >> Longevity regulating pathway - multiple species;Human Diseases >> Neurodegenerative disease >> Prion disease;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Genetic Information Processing >> Folding, sorting and degradation >> Protein processing in endoplasmic reticulum
Research Areas>>Spliceosome; / >>MAPK signaling pathway; / >>Protein processing in endoplasmic reticulum; / >>Endocytosis; / >>Longevity regulating pathway - multiple species; / >>Antigen processing and presentation; / >>Estrogen signaling pathway; / >>Prion disease; / >>Legionellosis; / >>Toxoplasmosis; / >>Measles; / >>Lipid and atherosclerosis
FunctionFunction:Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones.,induction:Constitutively synthesized.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the heat shock protein 70 family.,subcellular location:Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,subunit:Interacts with HSPH1/HSP105. Interacts with IRAK1BP1 (By similarity). Interacts with PACRG and TSC2. Interacts with SV40 VP1.,tissue specificity:Ubiquitous.,
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