Price range: $268.00 through $328.00
Background:
HSPs are a group of stress proteins induced by cells stimulated by stressors, which are related to tumorigenesis, proliferation and differentiation. According to their molecular weight, they can be divided into three types, and the distribution and function of HSPs in each group are different. HSP60 is mainly used for the study of colon cancer, tongue squamous cell carcinoma, pancreatic cancer and breast cancer, and may have prognostic significance.
| Size | 50μL/100μL |
| Target | Hsp60 |
| Reactivity | Human, Mouse, Rat |
| Applications | IHC, WB, IF, ELISA |
| MW(Calculated) | 60kD |
| MW(Observed) | 60kD |
| Host Species | Mouse |
| Isotype | IgG2b,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1000; WB 1:500-2000; IF 1:100-500; ELISA 1:1000-5000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein G affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RMM186 |
| Immunogen | Synthesized peptide derived from human HSP60 AA range: 500-573 |
| Specificity | The antibody can specifically recognize human HSP60 protein. In western blotting of K562, A431, MCF7 cell lysates, the antibody can label a 60 kDa band corresponding to HSP60. |
| Gene Name | HSPD1 |
| Protein Name | 60 kDa heat shock protein mitochondrial |
| Other Name | 60 kDa chaperonin; / 60 kDa heat shock protein, mitochondrial; / CH60_HUMAN; / Chaperonin 60; / Chaperonin, 60-KD; / CPN60; / fa04a05; / GROEL; / heat shock 60 kDa protein 1; / chaperonin; / Heat shock protein 1; / chaperonin; / Heat shock protein 60; / Heat shock protein 65; / heat shock protein family D; / Hsp60; / member 1; / HLD4; / Hsp 60; / HSP 65; / HSP-60; / HSP60; / HSP65; / HSPD1; / HuCHA60; / Mitochondrial matrix protein P1; / P60 lymphocyte protein; / short heat shock protein 60 Hsp60s1; / SPG13 |
| Species | Human |
| Gene ID | 3329 |
| Uniprot | P10809, |
| Species.1 | Mouse |
| Gene ID.1 | 15510 |
| Uniprot.1 | P63038, |
| Species.2 | Rat |
| Gene ID.2 | 63868 |
| Uniprot.2 | P63039 |
| Cellular Localization | Cytoplasmic |
| Tissue Expression | Cytoplasmic |
| Signaling_pathway | Genetic Information Processing >> Folding, sorting and degradation >> RNA degradation |
| Research Areas | >>RNA degradation; / >>Type I diabetes mellitus; / >>Legionellosis; / >>Tuberculosis; / >>Lipid and atherosclerosis |
| Function | Disease:Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.,Disease:Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first 2 decades of life.,Function:Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.,similarity:Belongs to the chaperonin (HSP60) family.,similarity:Belongs to the TCP-1 chaperonin family.,subunit:Interacts with HBV protein X and HTLV-1 protein p40tax., |
