Price range: $268.00 through $328.00
Background:
Topoisomerase IIα (Topo IIα) is a nuclear protein with a molecular weight of 170 kDa. It belongs to the topoisomerase family and is expressed in proliferating cells. The main function is to maintain the spatial conformation of DNA and ensure its function. It is mainly used for the study of drug resistance.
| Size | 50μL/100μL |
| Target | Topo IIα |
| Reactivity | Human, Mouse, Rat |
| Applications | IHC, WB, IF, ELISA |
| MW(Calculated) | 174kD |
| MW(Observed) | 174kD |
| Host Species | Mouse |
| Isotype | IgG1,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1000; WB 1:500-2000; IF 1:100-500; ELISA 1:1000-5000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein G affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RMM272 |
| Immunogen | Synthesized peptide derived from human Topoisomerase IIα AA range: 1400-1531 |
| Specificity | The antibody can specifically recognize human Topoisomerase IIα protein. |
| Gene Name | TOP2A TOP2 |
| Protein Name | DNA topoisomerase 2-alpha |
| Other Name | alpha isozyme; / ATP hydrolyzing DNA topoisomerase II alfa; / DNA gyrase; / DNA topoisomerase; / ATP hydrolyzing; / DNA topoisomerase 2 alpha; / DNA topoisomerase 2-alpha; / DNA topoisomerase II 170 kD; / DNA topoisomerase II alpha isozyme; / DNA topoisomerase II; / DNA Topoisomerase2; / TOP 2A; / TOP2; / TOP2A; / TOP2A_HUMAN; / Topoisomerase DNA II alpha 170 kDa; / TP2A |
| Species | Human |
| Gene ID | 7153 |
| Uniprot | P11388, |
| Species.1 | Mouse |
| Gene ID.1 | 21973 |
| Uniprot.1 | Q01320, |
| Species.2 | Rat |
| Gene ID.2 | 360243 |
| Uniprot.2 | P41516 |
| Cellular Localization | Nuclear |
| Tissue Expression | Expressed in the tonsil, spleen, lymph node, thymus, skin, pancreas, testis, colon, kidney, liver, brain and lung (PubMed:9155056). Also found in high-grade lymphomas, squamous cell lung tumors and seminomas (PubMed:9155056). |
| Signaling_pathway | |
| Research Areas | >>Platinum drug resistance |
| Function | Catalytic activity:ATP-dependent breakage, passage and rejoining of double-stranded DNA.,enzyme regulation:Specifically inhibited by the intercalating agent amsacrine.,Function:Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.,miscellaneous:Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.,PTM:Phosphorylation has no effect on catalytic activity.,similarity:Belongs to the type II topoisomerase family.,subcellular location:Generally located in the nucleoplasm.,subunit:Homodimer. Interacts with COPS5., |
